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Hepatocyte growth factor/scatter factor induces not only scattering but also cohort migration of human colorectal-adenocarcinoma cells


, : Hepatocyte growth factor/scatter factor induces not only scattering but also cohort migration of human colorectal-adenocarcinoma cells. International Journal of Cancer 78(6): 750-759, Dec 9

We presented earlier a 2-dimensional cell-motility ass using a highly metastatic variant (L-10) of human rectal adenocarcinoma cell line RCM-1 as a motility model of tumor cells of epithelial origin. In this model, L-10 cells moved coherent cell sheets when stimulated with 12-O-tetradecanoylphorbol-13-acetate (TPA), and we called this type of movement "cohort migration". Electron- and immuno electron-microscope study of the migrating cell sheets demonstrated localized release from cell-cell adhesion only at the lower portion of the cells with loss of E-cadherin immunoreactivity, and this change was associated with increased tyrosin phosphorylation of the E-cadherin-catenin complex, including beta-catenin. In the present study, to obtain evidence to support the relevance of our model to carcinoma-cell movement vivo, we sought a naturally occurring motogenic factor(s) able to induce this cohort migration. Among the factors examined, hepatocyte growth factor/scatter factor (HGF/SF) clearly induced cohort migration of L-10 cells. Additionally, not only L-10 but several other human colorectal-carcinoma cell line showed this type of migration in response to HGF/SF, while yet others showed scattering-type motility. In this HGF/SF induced migration, localized release from cell-cell adhesion was induced only at the lower portion of the cells, allowing them to extend leading lamellae, whereas close cell-cell contacts remained at the upper portion of the cells, as seen in TPA-induced cohort migration. Scattering-type cell lines tended to express more c-Met (receptor for HGF/SF) mRNA than the cell lines that showed cohort-type migration. LoVo, one of the scattering-type cell lines, expressed more c-Met protein and less E-cadherin than L-10, which showed cohort-type migration. HGF/SF treatment of LoVo reduced the amount of alpha-catenin complexed with E-cadherin more markedly than in L-10, but in both cell lines this reduction was not accompanied by increased tyrosine phosphorylation of beta-catenin, suggesting the presence of a mechanism other than phosphorylation for release from cell-cell adhesion during cell motility.

US$19.90

PMID: 9833769

DOI: 10.1002/(sici)1097-0215(19981209)78:6<750::aid-ijc13>3.0.co;2-#


Other references

Coleman, W.J.; Govindjee, 1987: A model for the mechanism of chloride activation of oxygen evolution in photosystem II. A hypothesis is proposed to explain the function of Cl(-) in activating the oxygenevolving complex (OEC) of photosystem II (PS II), based on the results of recent (35)Cl-NMR studies. The putative mechanism involves Cl(-) binding to two types of si...

Goodman A.L.; Neill J.D., 1975: Ovarian regulation of luteinizing hormone release in rabbits. Federation Proceedings 34(3): 287

Shevrin, H.; Smith, W.H.; Fritzler, D.E., 1970: Repressiveness as factor in subliminal activation of brain and verbal responses. Psychiatry Digest 31(7): 37-37

Rackemann, F.M., 2011: The nurse and the patient with asthma. American Journal of Nursing 47(7): 463-466

Cucolo, G.F.; Pearlman, H.S.; Ramachandran, V.S., 1964: Neurilemmoma Of Os Calcis. Approximately 10 cases of this type of tumor have been reported; this is the first report of its occurrence in the os calcis. The origin of neurogenic tissue appearing in the bone lesion is apparently from minute nerve twigs accompanying the nutri...

Koester, V., 1975: Housing preservation in the country. 12Mandsbladet 47 (11) 513-521

Va.D.Rp.A.W.V.; Deane.H.W., 1950: A morphological and cytochemical study of the postnatal development of the rat's adrenal cortex. Anatomical Record 107(3): 265-281

Chapelon, J.Y.; Margonari, J.; Vernier, F.; Gorry, F.; Ecochard, R.; Gelet, A., 1992: In vivo effects of high-intensity ultrasound on prostatic adenocarcinoma Dunning R3327. High-intensity ultrasound has been used to treat Dunning R3327 prostatic adenocarcinoma implanted s.c. in Fischer Copenhagen rats. Focused ultrasound was generated with a 1-MHz transducer and energy was provided by a 7.5-kW power amplifier. Sevent...

Barbour, A.D., 1929: Enzymatic hydrolysis of glycogen. The hydrolysis of glycogen may be readily carried out by means of a glycerol extract of fresh muscle or liver tissue. Such an extract does not diminish in potency on long standing in a refrigerator, and its activity is only diminished after incuba...

Kaitala, V.; Mappes, T.; Ylonen, H., 1997: Delayed female reproduction in equilibrium and chaotic populations. Behavioural and life history polymorphisms are often observed in animal populations. We analyse the timing of maturation and reproduction in risky and resource-limited environments. Field and laboratory evidence suggests that female voles and mice...