+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Homing endonucleases: Structure, function and evolution

, : Homing endonucleases: Structure, function and evolution. CMLS Cellular & Molecular Life Sciences 55(10): 1304-1326, Aug 15

'Homing' is the lateral transfer of an intervening genetic sequence, either an intron or an intein, to a cognate allele that lacks that element. The end result of homing is the duplication of the intervening sequence. The process is initiated by site-specific endonucleases that are encoded by open reading frames within the mobile elements. Several features of these proteins make them attractive subjects for structural and functional studies. First, these endonucleases, while unique, may be contrasted with a variety of enzymes involved in nucleic acid strand breakage and rearrangement, particularly restriction endonucleases. Second, because they are encoded within the intervening sequence, there are interesting limitations on the position and length of their open reading frames, and therefore on their structures. Third, these enzymes display a unique strategy of flexible recognition of very long DNA target sites. This strategy allows these sequences to minimize nonspecific cleavage within the host genome, while maximizing the ability of the endonuclease to cleave closely related variants of the homing site. Recent studies explain a great deal about the biochemical and genetic mechanisms of homing, and also about the structure and function of several representative members of the homing endonuclease families.


PMID: 10487208

DOI: 10.1007/s000180050372

Other references

Shibata, D.; Martin, W.J.; Arnheim, N., 1988: Analysis of DNA sequences in forty-year-old paraffin-embedded thin-tissue sections: a bridge between molecular biology and classical histology. DNA sequences from human tissues paraffin embedded 40 yr ago were studied using the in vitro gene amplification technique known as the polymerase chain reaction. Although significant DNA degradation was observed, single copy genomic sequences and...

Razavi, S.Mehdi.; Nazemiyeh, H.; Delazar, A.; Asnaashari, S.; Hajiboland, R.; Sarker, S.D.; Omidi, Y., 2011: Chemical variation of the essential oil of Prangos uloptera DC. at different stages of growth. In the current investigation, the influence of the plant growth stages (prior to emergence of flower heads or vegetative, anthesis and fruiting) on the essential oil content and composition in the aerial parts of Prangos uloptera DC. was studied....

McEntegart, D., 2011: A relevant question for site balanced studies relates to the comparative predictability of minimization to the alternative of permuted blocks. Contemporary Clinical Trials 31(6): 507-507

Bauman, L.A.; Kish, I.; Baumann, R.C.; Politis, G.D., 1999: Pediatric sedation with analgesia. Sedation with analgesia is frequently required to perform painful or invasive procedures in children. The best medication combination for pediatric sedation with analgesia is yet to be identified. Sixty-four of 243 total sedation with analgesia pr...

Wahaab, R.A., 2000: Biological degradation of some organic compounds enrolled in paper industry--a pollution prevention approach. Evaluation of the elimination and the "ultimate" biodegradation by aerobic microorganisms of some organic compounds commonly used in paper manufacturing technology was investigated. Biodegradation lines of nine organic compounds were det...

Manfredini, R.; Boari, B.; L.C.cilia, O.; Steliu, J.; Salmi, R.; Michelini, V.; Zanotti, C.; Bigoni, M.; Gallerani, M.; Fersini, C.; Portaluppi, F., 2001: Circadian pattern of emergency calls. Chronobiology International 18(6): 1158-1159, November

Shiflinger, L.E., 1956: Casting out of the separated after-birth. Akusherstvo i Ginekologiia 32(1): 72-74

Fishman J.A., 1988: Cross reactive antigens of rat and human pneumocystis carinii a western immunoblot analysis. Clinical Research 36(3): 455A

Zhang, Y.; Pignatello, J.J.; Tao, S.; Xing, B., 2015: Bioacessibility of PAHs in fuel soot assessed by an in vitro digestive model: effect of including an absorptive sink. Polycyclic aromatic hydrocarbons (PAHs) associated with soot or black carbon can enter the human digestive tract by unintentional ingestion of soil or other particles. This study investigated the bioaccessibility of 11 PAHs in a composite fuel soo...

Mezzano, S.; Aros, C., 2005: Chronic kidney disease: classification, mechanisms of progression and strategies for renoprotection. Chronic kidney disease is a worldwide health problem. The incidence and prevalence of kidney failure is in constant increase, involving poor outcomes and high costs. The leading causes of kidney failure are type 2 diabetes and hypertension. The ne...