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Identification of Candida albicans by using milk Tween agar

, : Identification of Candida albicans by using milk Tween agar. Zhonghua Pifuke Zazhi 32(5): 309-312, Oct

Objective: To improve the method of identifying Candida albicans. Methods: A new medium consisting of milk powder, Tween 80 and agar (milk tween agar, MTA) was designed for the development of both germ tube and chlamydoconidia by C. albicans. In total 103 isolates, including C. albicans (88), C. guilliermondii (3), C. krusei (3), C. parapsilosis (3), C. tropicalis (3), and C. keytr (3) were cultured on this medium, compared with those on corn meal tween agar medium or inactivated human sera. Results: The germ tube production by C. albicans was seen in 69 out of 88 strains on MTA medium. C. albicans was found to be able to produce a higher percentage of chlamydoconidia (85.2%) after 24 hour incubation in MTA medium than in the conventional corn meal tween 80 medium (46.6%). Conclusion: The new medium gives a signficantly higher percentage of germ tube and chlamydoconidia production, and can be used in the clinical mycology laboratory.


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