+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of zeta-crystallin/NADPH:quinone reductase as a renal glutaminase mRNA pH response element-binding protein

, : Identification of zeta-crystallin/NADPH:quinone reductase as a renal glutaminase mRNA pH response element-binding protein. Journal of Biological Chemistry 276(24): 21375-21380

Increased renal ammoniagenesis and bicarbonate synthesis from glutamine during chronic metabolic acidosis facilitate the excretion of acids and partially restore normal acid-base balance. This adaptation is sustained, in part, by a cell-specific stabilization of the glutaminase mRNA that leads to an increased synthesis of the mitochondrial glutaminase. A direct repeat of an 8-base AU sequence within the 3'-nontranslated region of the glutaminase mRNA binds a unique protein with high affinity and specificity. Expression of various chimeric mRNAs in LLC-PK(1)-FBPase(+) cells demonstrated that a single 8-base AU sequence is both necessary and sufficient to function as a pH response element (pH RE). A biotinylated oligoribonucleotide containing the direct repeat was used as an affinity ligand to purify the pH RE-binding protein from a cytosolic extract of rat renal cortex. The purified binding activity retained the same specific binding properties as observed with crude extracts and correlated with the elution of a 36-kDa protein. Microsequencing by mass spectroscopy and Western blot analysis were used to identify this protein as zeta-crystallin/NADPH:quinone reductase. The purified protein contained eight tryptic peptides that were identical to sequences found in mouse zeta-crystallin and three peptides that differed by only a single amino acid. The observed differences may represent substitutions found in the rat homolog. A second protein purified by this protocol was identified as T-cell-restricted intracellular antigen-related protein (TIAR). However, the purified TIAR neither bound nor affected the binding of zeta-crystallin/NADPH:quinone reductase to the pH RE. Furthermore, specific antibodies to zeta-crystallin, but not TIAR, blocked the formation of the complex between the pH RE and either the crude cytosolic extract or the purified protein. Thus, zeta-crystallin/NADPH:quinone reductase is a pH response element-binding protein.


PMID: 11294877

DOI: 10.1074/jbc.M101941200

Other references

Peixoto, J., R.; Ferraz, F., M.; Santos, L., C.; Angelis, B.-De; Juliatti, F., C., 1998: Screening of sweet potato genotypes resistant to the rood-knot nematode (Meloidgyne spp.). A greenhouse screening at the Universidade Federal de Uberlandia was conducted to select sweet potato genotypes resistant to Meloidogyne incognita and M. javanica. The experimental design was randomized blocks, with four replications in a split pl...

Zhao, Z.Y.; Lee, C.C.; Jiralerspong, S.; Juyal, R.C.; Pandolfo, M.; Chinault, A.C.; Greenberg, F.; Patel, P.I.; Caskey, C.T., 1994: Analysis of two novel cDNAs from the Smith-Magenis syndrome region on chromosome 17. American Journal of Human Genetics 55(3 SUPPL ): A275

Reinshagen, M.; Engele, J.; Lakshmanan, J.; Geerling, I.; Rohm, H.; Eysselein, V.E., 1998: Expression of GDNF, RET, GFR-alpha1/GFR-alpha2 during the timecourse of experimental colitis in the rat. Society for Neuroscience Abstracts 24(1-2): 798

Jaafar, M.; Yanes, R.; Asenjo, A.; Chubykalo-Fesenko, O.; Vázquez, M.; González, E.M.; Vicent, J.L., 2008: Field induced vortex dynamics in magnetic Ni nanotriangles. The magnetization states in Ni triangular dots under an applied magnetic field have been studied using variable-field magnetic force microscopy (VF-MFM) imaging. In order to understand their dynamics we performed micromagnetic simulations which ar...

Yokoyama, T., 1975: Pestalotia distincta refound in Japan. Mycologia 67 (5) 1032-1035

Goodbred Steven L.J.; Kuehl Steven A., 1999: Holocene and modern sediment budgets for the Ganges-Brahmaputra river system; evidence for highstand dispersal to flood-plain, shelf, and deep-sea depocenters. The partitioning of fluvial sediment load across continental margins is an important control on strata formation and sequence development; however, few quantitative sediment budgets that encompass entire dispersal systems exist. For the Ganges-Bra...

Saade, G.R.; Moise, K.J.; Belfort, M.A.; Hesketh, D.E.; Carpenter, R.J., 1993: Fetal and neonatal hematologic parameters in red cell alloimmunization: predicting the need for late neonatal transfusions. The purpose of this study was to determine whether the need for late neonatal transfusion can be predicted in fetuses who received intrauterine transfusions. The records of 36 patients who underwent intrauterine transfusion were received. Newborns...

Gordon, D.M.; Hunter, B.; Woods, L.N.; Tinney, B.; Bostic, B.; Malone, S.; Kimbro, G.; Greenlee, D.; Fabish, S.; Harris, K.; Smith, A., 2012: Increasing Outreach, Connection, and Services to Low-Income Non-Custodial Fathers: How Did We Get Here and What Do We Know. This paper documents a model for outreaching, connecting, and serving low-income, ethnically diverse, non-custodial fathers. Men are engaged "where they are" by building their strengths and addressing their needs. The Male Involvement Ne...

Takeda, T.; Kitahara, M., 1989: Compensation of otolith function. Horizontal eye displacement in total darkness during lateral tilting about the body axis was examined in 14 patients with unilateral loss of labyrinth function, including 8 cases of positional vertigo or dizziness and 6 symptom-free cases. The cha...

Lemon, W.C.; Getz, W.M., 2001: Rate code input produces temporal code output from cockroach antennal lobes. The experiments presented here were designed to determine the origin of the temporally complex activity of antennal lobe projection neurons in the cockroach olfactory system. We determined this through the use of complex chemical stimuli that evok...