geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunolocalization of G(alpha)i-3 in foetal lung fibroblasts


, : Immunolocalization of G(alpha)i-3 in foetal lung fibroblasts. European Respiratory Journal 12(5): 1137-1140

Guanosine triphosphate (GTP)-binding proteins or G proteins are involved in a wide variety of well-recognized signalling activities between cell surface receptors and effectors. The heterotrimeric G proteins have alpha,beta and gamma subunits organized in a trimeric structure. The aim of this study was to localize G(alpha)i-3, an important heterotrimeric G protein, in foetal lung cells. Using a foetal lung fibroblast cell line (RFL-6), the localization of G(alpha)i-3 was determined by immunofluorescence using a specific antibody to G(alpha)i-3, colocalization with a lectin known to bind the Golgi complex and Western blotting of RFL-6 cellular membrane proteins. This study identified G(alpha)i-3 on the Golgi membranes in rat foetal lung cells. Treatment with cycloheximide, to block protein synthesis, diminished the cytosolic distribution of the protein, but intense Golgi staining remained. G(alpha)i-3, therefore, appears to be part of the Golgi complex and not present transiently during protein synthesis. In the nonpolar foetal lung fibroblasts studied, the intracellular concentration of G(alpha)i-3 suggests a role for this protein in the intracellular trafficking and regulation of proteins needed for normal lung development.

US$29.90

PMID: 9864010


Other references

Freitas, A.C.C.; Ximenes, N.C.A.; Aguiar, J.S.; Nascimento, S.C.; Lins, T.U.L.; Magalhães, L.R.; Coelho, L.C.B.B.; Carneiro-da-Cunha, M.G.; Gonçalves-Silva, T.; Correia, M.T.S., 2012: Biological Activities of Libidibia (Caesalpinia) ferrea var. parvifolia (Mart. ex Tul.) L. P. Queiroz Pod Preparations. Libidibia ferrea has been used in folk medicine throughout Brazil, and this study evaluated the biological activities of crude extract (CE) as well as a partially purified fraction (F80) obtained from its pods. Results from the MTT assay revealed...

Smith, M., 1983: A team approach to health care. Nursing Outlook 31(5): 271, 273-271, 273

Stanco, P., 1987: Breed a cory. Tropical Fish Hobbyist, 364: 62, 64-66 No. 382

Harper, A.D., 1985: Spaced-roof ventilation at West Learmonth. Re-roofing of a building for finishing cattle with spaced replacement roof sheeting is described. Coloured aluminium roofing (0.5 mm thick) was laid with continuous 19 mm gaps between the sheets on both roof slopes. Each length runs in 1 piece fro...

Robertson, K.M.; Johnson, D.L., 2004: Vertical redistribution of pebbles by crayfish in mollisol catenas of Central Illinois. Soil mixing by organisms (bioturbation) has an important influence on the distribution of rock fragments in soils. In east-central Illinois, pebbles were observed on the surfaces of loessal catenas. We hypothesized that surface pebbles are brought...

Sturhan, D., 1976: Outdoor occurrence of Meloidogyne species in Western Germany. A survey in the German Federal Republic showed 4 species of Meloidogyne. M. hapla was widespread and the most common and preferred lighter soils. It was found on Ligustrum vulgare, Convolvulus arvensis, Rumex hydrolapathum and R. obtusifolius whic...

Iranmanesh, A.; Mulligan, T.; Veldhuis, J.D., 2010: Age in men does not determine gonadotropin-releasing hormone's dose-dependent stimulation of luteinizing hormone secretion under an exogenous testosterone clamp. Aging is associated with a decline in incremental LH pulse amplitude, which could be due to decreased GnRH secretion or impaired GnRH action. Inconsistent published studies of GnRH action in older men may be due to disparate sex-steroid milieus. T...

Sirbu, Z., 1966: Electron microscopic changes in lymph nodes of pigs experimentally infected with swine fever. Intranuclear accumulations of electron-dense particles 75-130 A diam. were present in lymph node cells from 16 pigs inoculated i/m with the virus. Degenerative changes were present in the nuclei of reticular cells; changes in the cytoplasm were le...

Saely, C.H.; Vonbank, A.; Drexel, H., 2010: HDL cholesterol and residual risk of first cardiovascular events. Lancet 376(9754): 1738; Author Reply 1738-9

Spek, J.C.; Heijna, B.J., 1976: Double glazing critically evaluated. Aspects of greenhouse double-glazing examined include types, quality criteria, depreciation, light permeability and heat saving.