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Information transfer between large and small two-dimensional polyacrylamide gel electrophoresis


, : Information transfer between large and small two-dimensional polyacrylamide gel electrophoresis. Electrophoresis 20(18): 3508-3513

To determine the feasibility of data transfer, an interlaboratory comparison was conducted on colon carcinoma cell line (DLD-1) proteins resolved by two-dimensional polyacrylamide gel electrophoresis either on small (6 X 7 cm) or large (16X18 cm) gels. The gels were silver-stained and scanned by laser densitometry, and the image obtained was analyzed using Melanie software. The number of spots detected was 1337+-161 vs. 2382 +- 176 for small vs. large format gels, respectively. After gel calibration using landmarks determined using pl and Mr markers, large- and small-format gels were matched and 712+-36 proteins were found on both types of gels. Having performed accurate gel matching it was possible to acquire additional information after accessing a 2-D PAGE reference database (http://www.expasy.ch/ cgibin/map2/ def?DLD1-HUMAN). Thus, the difference in gel size is not an obstacle for data transfer. This will facilitate exchanges between laboratories or consultation concerning existing databases.

US$19.90

PMID: 10612276

DOI: 10.1002/(SICI)1522-2683(19991201)20:18<3508::AID-ELPS3508>3.0.CO;2-7


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