geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Interaction between RNA polymerase and RapA, a bacterial homolog of the SWI/SNF protein family


, : Interaction between RNA polymerase and RapA, a bacterial homolog of the SWI/SNF protein family. Journal of Biological Chemistry 275(29): 22090-7

Recently, we identified a novel Escherichia coli RNA polymerase (RNAP)-associated protein, an ATPase, called RapA (Sukhodolets, M. V. , and Jin, D. J. (1998) J. Biol. Chem. 273, 7018-7023). RapA is a bacterial homolog of SWI2/SNF2. We showed that RapA forms a stable complex with RNAP holoenzyme and that binding to RNAP holoenzyme stimulates the ATPase activity of RapA. We have further analyzed the interactions between purified RapA and the two forms of RNAP: core RNAP and RNAP holoenzyme. We found that RapA interacts with either form of RNAP. However, RapA exhibits higher affinity for core RNAP than for RNAP holoenzyme. Chemical cross-linking of the RNAP-RapA complex indicated that the RapA-binding sites are located at the interface between the alpha and beta' subunits of RNAP. Contrary to previously reported results (Muzzin, O., Campbell, E., A., Xia, L., Severinova, E., Darst, S. A., and Severinov, K. (1998) J. Biol. Chem. 273, 15157-15161), our in vivo analysis of a rapA null mutant suggested that RapA is not likely to be directly involved in DNA repair.

US$19.90

PMID: 10801781

DOI: 10.1074/jbc.M000056200


Other references

Tari A.; Huang L., 1989: Bilayer stabilization activity of phosphatidylglycerol in dioleoylphosphatidylethanolamine liposomes. Biophysical Journal 55(2 PART 2): 105A

Hornová, J., 1999: Trabeculectomy with releasable sutures and corneal topography. We are interested in the influence of trabeculectomy (TE) with releasable sutures to corneal curvature in the early postoperative period. 35 eyes of 31 patients were followed after operation. Corneal topography was made on the Topograph Opticon 20...

Fan, E.; Needham, D.M.; Stewart, T.E., 2005: Ventilatory management of acute lung injury and acute respiratory distress syndrome. Context The acute lung injury and acute respiratory distress syndrome are critical illnesses associated with significant morbidity and mortality. Mechanical ventilation is the cornerstone of supportive therapy. However, despite several important a...

Geiser, M.H.; Diermann, U.; Riva, C.E., 1999: Compact laser Doppler choroidal flowmeter. A compact instrument is described that allows the measurement of the laser Doppler flow parameters, i.e., the velocity, the volume, and flow of blood in the foveal region of the human choroidal vascular system. This new device uses the optical pri...

Hodgson John H., 1962: Movements in the earths crust as indicated by earthquakes. Reviews the available seismic data relating to the mechanism and sense of movement in the circum-Pacific and the east Indies-central Asia-south Europe tectonic zones, with particular reference to interpretations based on a fault model and on a col...

Amirghofran, Z.; Sheikhi, A.K.; Kumar, P.V.; Saberi Firouzi, M., 2002: Soluble HLA class I molecules in malignant pleural and peritoneal effusions and its possible role on NK and LAK cytotoxicity. Purpose: To examine the amount of sHLA-I in malignant pleural and peritoneal effusions and its possible role in natural immune defense. Methods: Three groups of patients (75 patients with malignancy, 21 with infection, and 27 with other diseases)...

McInerney, C., 1999: Nurses and politics: politically correct combination. Nursing Spectrum 8(22): 18-19

Toygarli, S.A., 1956: Melanosarcoma in horses, a donkeyand a mule: report of nine cases. Brief case reports, with some illustrations, are given of melanomas, some of which metastasized, in 7 horses (six grey, one brown), a donkey (right upper eyelid of a 10-year-old dark-brown animal) and a mule (anterior thoracic region of a 10-year-...

Adler, K.A.; Mills, P.J.; Dimsdale, J.E.; Ziegler, M.G.; Patterson, T.L.; Sloan, R.P.; Grant, I., 2002: Temporal stability of acute stress-induced changes in leukocyte subsets and cellular adhesion molecules in older adults. This study investigated the temporal stability of enumerative immune and catecholamine responses to acute psychosocial stress in 67 Alzheimer's caregivers ages 56-82 years (45 women and 22 men) who were required to prepare and deliver two 3-m...

Jacobson, N., 2010: Dignity violation in health care. In this grounded theory analysis I sought to understand dignity violation in health care and to explore the context in which such violations take place. I found that dignity violation in health care occurs through processes of rudeness, indifferen...