geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Isolation and identification of the related gene mediating gamma-radiation-induced apoptosis in human HL-60 cells


, : Isolation and identification of the related gene mediating gamma-radiation-induced apoptosis in human HL-60 cells. Xi'an Yike Daxue Xuebao 20(3): 293-296, Sept

To identify the member of the caspase family proteases involved in gamma-radiation-induced apoptosis in HL-60 cells, using degenerated oligonucleotide primers encoding the highly conserved peptides, which were present in all known caspases, RT-PCR was performed on poly (A) RNA from the gamma-radiation-induced apoptotic HL-60 cells. Then, cloned and sequenced to identify the amplified DNA fragments. The results showed that the amplified DNA fragments were identified with a part of caspase-3 cDNA. It indicated that caspase-3 was involved in gamma-radiation-induced apoptosis in HL-60 cells and may be the pivotal element of radiation-induced apoptosis.

US$29.90


Other references

Skager, A.A.; Linare, A.R.; Barkane, B.Ia.; Kandaurova, V.F.; Nemtsev, M.A., 1984: Prevention of regenerative disorders in wounds and fractures of the maxillofacial area. Stomatologiia 63(3): 47-50

Kellner, U.; Den Hollander, A.I.; D.K.k, Y.J.M.; Cremers, F.P.M.; Kraus, H.; Foerster, M.H., 2000: Clinical findings in patients with RP12 and mutations in the CRB1 gene. IOVS 41(4): S885, March 15

Faria, L.C.; Gu, F.; Parada, I.; Barres, B.; Luo, Z.David.; Prince, D.A., 2017: Epileptiform activity and behavioral arrests in mice overexpressing the calcium channel subunit α2δ-1. The alpha2delta-1 subunit (α2δ-1) of voltage-gated calcium channels is a receptor for astrocyte-secreted thrombospondins that promote developmental synaptogenesis. Alpha2delta-1 receptors are upregulated in models of injury-induced peripheral pa...

Pan, Z.; Tian, P.; Zou, H.; Liu, X.; Xie, C.; Zhu, L., 2015: The recurrent rate of nasal endoscopic microsurgical skills for the treatment of nasal inverted papilloma: a meta-analysis. To investigate the recurrence rate of nasal inverted papilloma treating by endoscopic and non-endoscopic approach. A search on Pubmed, Medline, Springer and Elsevier databases was done to collect the reports (2001-2013) concerning different surger...

Buckley, FG.; Buckley, PA., 1979: Do Aleutian terns exhibit extraordinary antipredator adaptations. Proceedings of the Conference of the Colonial Waterbird Group, 3: 99-107

Davis, G.L.; Roberts, W.L., 2010: The healthcare burden imposed by liver disease in aging Baby Boomers. The Baby Boomer generation is composed of 78 million Americans who are just beginning to reach their retirement years. Most Boomers have at least one chronic health problem, and these significantly increase the expense of providing medical care. L...

Joens Heinz Peter, 1983: Large-scale structural classification of the surface of Mars. Proceedings of the International Conference on Basement Tectonics 4: 359-367

Mironovich, N.I., 1964: Some Clinical And Therapeutical Problems Concerning Acute Inflammatory Processes Following Surgery Of The Posterior Cranial Fossa In Children. Voprosy Neirokhirurgii 28: 32-36

Carlin, K.P.; Jiang, Z.; Brownstone, R.M., 1997: Calcium currents in functionally mature mouse spinal cord motoneurones. Society for Neuroscience Abstracts 23(1-2): 1302

Cowling, D.W., 1961: The effect of white clover and nitrogenous fertilizer on the production of a sward. 1. Total annual production. Three-year leys of cocksfoot/white clover and cocksfoot alone were dressed with 0, 35, 105 and 210 lb N/ac every year to provide information on the effect of clover and N on annual production. White clover contributed 40% of the dry-matter yield b...