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Isolation and identification of the related gene mediating gamma-radiation-induced apoptosis in human HL-60 cells

, : Isolation and identification of the related gene mediating gamma-radiation-induced apoptosis in human HL-60 cells. Xi'an Yike Daxue Xuebao 20(3): 293-296, Sept

To identify the member of the caspase family proteases involved in gamma-radiation-induced apoptosis in HL-60 cells, using degenerated oligonucleotide primers encoding the highly conserved peptides, which were present in all known caspases, RT-PCR was performed on poly (A) RNA from the gamma-radiation-induced apoptotic HL-60 cells. Then, cloned and sequenced to identify the amplified DNA fragments. The results showed that the amplified DNA fragments were identified with a part of caspase-3 cDNA. It indicated that caspase-3 was involved in gamma-radiation-induced apoptosis in HL-60 cells and may be the pivotal element of radiation-induced apoptosis.


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