geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Isolation of novel beer-spoilage bacteria from the brewery environment


, : Isolation of novel beer-spoilage bacteria from the brewery environment. Journal of the American Society of Brewing Chemists 56(3): 114-117

Two strains of beer-spoilage bacteria, BS-1 and EM-63, were isolated from the environment (from drainage) in our breweries. Strain BS-1 belonged to a homo-fermentative species of the genus Lactobacillus. The partial sequence of 16S rDNA indicated that strain BS-1 was related to Lactobacillus coryniformis. However, the fermentation pattern of the sugars was different from that of L. coryniformis and other species of Lactobacillus, because of the narrow fermentation spectrum, glucose, mannose, and fructose. Strain EM-63 was a Gram-negative, strictly anaerobic rod-shaped bacterium with no flagella. The GC content of DNA was 41%, and the cell wall peptidoglycan was directly cross-linked meso-diaminopimelic acid. The major fermentation acids were succinic acid and D-lactic acid in the culture media, while propionic acid was neither produced in culture media nor in beer. These characteristics indicated that strain EM-63 did not belong to the genus Pectinatus, known as Gram-negative, rod-shaped, beer-spoilage bacteria, or the genus Zymophilus or Selenornonas, known as Gram-negative, rod-shaped, pitching yeast contaminants. On the basis of the physiological characteristics and genetic analysis, we conclude that these strains are novel beer-spoilage bacteria.

US$29.90


Other references

Hoffer, P.T.; Hoffer, A.; Freud, S.; Ferenczi, S., 2000: Ferenczi's fatal illness in historical context. In August 1932, Ferenczi was already manifesting symptoms of his fatal illness, pernicious anemia. This was diagnosed between September 20 and October 2 of that year, less than a month after the Twelfth International Psychoanalytic Congress in Wie...

Grivell, R.M.; Alfirevic, Z.; Gyte, G.Ml.; Devane, D., 2010: Antenatal cardiotocography for fetal assessment. Cardiotocography (CTG) is a continuous recording of the fetal heart rate obtained via an ultrasound transducer placed on the mother's abdomen. CTG is widely used in pregnancy as a method of assessing fetal well-being, predominantly in pregnan...

Gray H.B.; Coyle C.L.; Dooley D.M.; Grunthaner P.J.; Hare J.W.; Holwerda R.A.; Mcardle J.V.; Mcmillin D.R.; Rawlings J.; E.A., 1977: Structure and electron transfer reactions of blue copper proteins. Raymond, Kenneth N (Ed ) Advances in Chemistry Series, No 162 Bioinorganic Chemistry-Ii A Symposium at The 171st Meeting Of The American Chemical Society New York, N Y , Usa, April 7-9, 1976 Viii+448p Illus American Chemical Society: Washington, D C , Usa Isbn 0-8412-0359-8 145-155

Nicholas, J.; Coles, L.S.; Newman, C.; Honess, R.W., 1991: Regulation of the herpesvirus saimiri (HVS) delayed-early 110-kilodalton promoter by HVS immediate-early gene products and a homolog of the Epstein-Barr virus R trans activator. We have reported previously the detection of two stable immediate-early (IE) transcripts that accumulate in cycloheximide-treated cells infected with herpesvirus saimiri (HVS). These are the 1.6-kb mRNA from the 52-kDa gene (which is homologous to...

Wanty, R.B.; Goldhaber, M.B.; Morrison, J.M.; Lee, L., 2009: Regional variations in water quality and relationships to soil and bedrock weathering in the southern Sacramento Valley, California, USA. Regional patterns in ground- and surface-water chemistry of the southern Sacramento Valley in California were evaluated using publicly available geochemical data from the US Geological Survey's National Water Information System (NWIS). Within...

Johnson M.H., 1984: Mechanisms for generating and stabilizing cell asymmetries. Journal of Embryology & Experimental Morphology 82(SUPPL): 156

Casati, A.; Muttini, S.; Leggieri, C.; Colombo, S.; Giorgi, E.; Torri, G., 1998: Rapid turnover proteins in critically ill ICU patients. Negative acute phase proteins or nutritional indicators?. Rapid turnover proteins are often used as metabolic indicators in patients receiving nutritional therapy. However, their plasmatic concentration can be influenced by activation of acute phase reaction due to stress. The aim of this prospective, ob...

Hiroi, T.; Kobayashi, N.; Kuwabara, R.; Nishikawa, H.; Masuda, Y.; Matsui, H., 2002: Transcriptional regulation of the human serotonin 4 receptor gene. The human serotonin-4 receptor (5-HT4R) are thought to be involved in various central and peripheral pathological conditions, such as anxiety, mood disorders,neurodegenerative disorders, atrial arrhythimia and functional bowel syndrome. 5-HT4R gen...

Ismail; Soultani; Ghoul, 1998: Optimization of the enzymatic synthesis of butyl glucoside using response surface methodology. Enzymatic synthesis of butyl glucoside by beta-glucosidase from bitter almonds was optimized using response surface methodology (RSM). Empirical models were developed to describe relationships between the operating variables (temperature, water/bu...

Mukhopadhyay, D.; Gokulkrishnan, L.; Jones, P.; Mohanaruban, K., 2002: Audit of anticoagulation control: a comparison between the performance of a hospital anticoagulation clinic and the general practice. Clinical Medicine 2(2): 169-169