+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Late mitotic failure in mice lacking Sak, a polo-like kinase

, : Late mitotic failure in mice lacking Sak, a polo-like kinase. Current Biology 11(6): 441-446, 20 March

Polo-like kinases in yeast, flies, and mammals regulate key events in mitosis. Such events include spindle formation at G2/M, the anaphase-promoting complex (APC) at the exit from mitosis, the cleavage structure at cytokinesis, and DNA damage checkpoints in G2/M. Polo-like kinases are distinguished by two C-terminal polo box (pb) motifs, which localize the enzymes to mitotic structures. We previously identified Sak, a novel polo-like kinase found in Drosophila and mammals. Here, we demonstrate that the Sak kinase has a functional pb domain that localizes the enzyme to the nucleolus during G2, to the centrosomes in G2/M, and to the cleavage furrow during cytokinesis. To study the role of Sak in embryo development, we generated a Sak null allele, the first polo-like kinase to be mutated in mice. Sak(-/-) embryos arrested after gastrulation at E7.5, with a marked increase in mitotic and apoptotic cells. Sak(-/-) embryos displayed cells in late anaphase or telophase that continued to express cyclin B1 and phosphorylated histone H3. Our results suggest that Sak is required for the APC-dependent destruction of cyclin B1 and for exit from mitosis in the postgastrulation embryo.


PMID: 11301255

DOI: 10.1016/s0960-9822(01)00117-8

Other references

Blinkenberg, M.; Akeson, P.; Sillesen, H.; Lövgaard, S.; Sellebjerg, F.; Paulson, O.B.; Siebner, H.R.; Sørensen, P.S., 2013: Chronic cerebrospinal venous insufficiency and venous stenoses in multiple sclerosis. The traditional view that multiple sclerosis (MS) is an autoimmune disease has recently been challenged by the claim that MS is caused by chronic cerebrospinal venous insufficiency (CCSVI). Although several studies have questioned this vascular th...

Shenker B.J.; Rooney C.; Vitale L.; Shapiro L.M., 1992: Suppression of human t and b cell activation by mercuric compounds. Journal of Dental Research 71(SPEC ISSUE): 625

Nichelmann, M.; Lyhs, L., 1968: Consenusal vascular reactions in pigs. IV. Consensual vascular reactions of the skin after cooling of the intestine. Archiv für Experimentelle Veterinarmedizin 22(3): 615-619

Fiveg M.P., 1948: O godovom tsikle sedimentatsii kamennoi soli Verkhnekamskogo mestorozhdeniya. The Kungurian (Permian) rock salt deposits of the upper Kama river region, USSR, exhibit an alternation of layers which is attributed to seasonal cycles of deposition. Although the deposits have been subjected to recrystallization during tectonic...

Polk, H.C., 2004: A Hamblin Letton Lecture. The continuing need to attract surgery students to surgical specialty careers: a step forward but at what expense?. American Surgeon 70(5): 379-383

Chen, M.; Dasgupta, C.; Xiong, F.; Zhang, L., 2014: Epigenetic upregulation of large-conductance Ca2+-activated K+ channel expression in uterine vascular adaptation to pregnancy. Our previous study demonstrated that pregnancy increased large-conductance Ca(2+)-activated potassium channel β1 subunit (BKβ1) expression and large-conductance Ca(2+)-activated potassium channel activity in uterine arteries, which were abrogate...

Komiya, Y.; Yoshida, Y.; Tomimura, T., 1960: The excretory system, particularly its flame cell pattern of Paragonimus iloktsuenensis Chen, 1940. Komiya et al. describe and figure the excretory system of the larval forms of Paragonimus iloktsuenensis. The flame cell pattern of the daughter rediae is represented as 2, and that of the cercariae and metacercariae as 2. The authors compare the...

Song, Y.M.; Kim, K.I.; Kim, C.S.; Choi, S.H.; Yoon, Y.H., 2000: Genotyping and antibiotic susceptibility of Streptococcus uberis. Raw milk and environmental specimens from 9 dairy farms in Kyunggido, Korea Republic were examined for S. uberis. Genotyping by pulsed field gel electrophoresis (PFGE) of Sma I digested genomes was performed to elucidate contamination route of raw...

Bent, J.P.; Dinges, D.; Whitehouse, A., 1992: Pathologic quiz case 1. Minor salivary gland pleomorphic adenoma of the parapharyngeal space. Archives of Otolaryngology--Head & Neck Surgery 118(6): 664-666

Davis, L.E.; Hofmann, W., 1969: A long-term nasogastric feeding tube made from modified Penrose tubing. JAMA 209(5): 685-686