+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

MGA2 is involved in the low-oxygen response element-dependent hypoxic induction of genes in Saccharomyces cerevisiae

, : MGA2 is involved in the low-oxygen response element-dependent hypoxic induction of genes in Saccharomyces cerevisiae. Molecular and Cellular Biology 21(18): 6161-6169

Eukaryotes have the ability to respond to changes in oxygen tension by alterations in gene expression. For example, OLE1 expression in Saccharomyces cerevisiae is upregulated under hypoxic conditions. Previous studies have suggested that the pathway regulating OLE1 expression by unsaturated fatty acids may involve Mga2p and Spt23p, two structurally and functionally related proteins. To define the possible roles of each of these genes on hypoxia-induced OLE1 expression, we examined OLE1 expression under normoxia, hypoxia, and cobalt treatment conditions in DELTAmga2 or DELTAspt23 deletion strains. The results of OLE1 promoter-lacZ reporter gene and Northern blot analyses showed that hypoxia- and cobalt-induced OLE1 expression was dramatically decreased in a DELTAmga2 strain but not in a DELTAspt23 strain. Further analyses using low-oxygen response element (LORE)-CYC1-lacZ fusion reporter assays and electrophoretic mobility shift assays (EMSAs) demonstrated that MGA2 significantly affects the LORE-dependent hypoxic induction pathway of gene expression. When MGA2 was supplied by a plasmid, the LORE-dependent hypoxia-inducible reporter expression was recovered, as was the hypoxia-inducible complex in EMSAs in the S. cerevisiae DELTAmga2 strain. Supershift analysis of EMSAs using crude extracts containing mycMga2p indicated that Mga2p is a component of the LORE-binding complex. Another LORE-dependent, hypoxia-inducible gene, ATF1, was similarly affected in the DELTAmga2 strain. These results indicate that MGA2 is required for the LORE-dependent hypoxic gene induction in S. cerevisiae.


PMID: 11509659

DOI: 10.1128/mcb.21.18.6161-6169.2001

Other references

Lin-Jing; Zhou-Wei-Chuan; Chen-De-Niu, 2007: A new species of the genus Xesta albers, 1850 from China (Pulmonata, Stylommatophora, Ariophantidae). A new speices of genus Xesta is described m the present paper. The studied materials were collected by Prof. XU Sheng Quan from Wengong Temple, Taibaishan (33 degrees 9' N, 107 degrees 7' E), Qinling, Shaanxi Province, China, 8 May, 200...

Barton Paul; Bethke P.; Wetlaufer Pamela Heald; Foley Nora; Hayba D.; Goss James, 1982: Silver/ base metal epithermal deposits. Open-File Report - U S

Kreisler, L., 1986: The practitioner confronted with present-day psychological and social emergencies in childhood and adolescence. La Revue du Praticien 36(25): 1421-1429

Spadella, C.T.; Suarez, O.A.X.; Lucchesi, A.N.; Marques, S.F.G.; Cataneo, A.J.M., 2011: Effects of pancreas transplantation on oxidative stress in pulmonary tissue from alloxan-induced diabetic rats. There is considerable evidence that cellular oxidative stress caused by hyperglycemia plays an important role in the genesis and evolution of chronic diabetic lesions. In this study, we determined the effectiveness of pancreas transplantation (PT)...

Wei, M.; Ren, H.; Yin, L., 2016: The combinational use of dual microcatheter technique and new hypersoft helical coil for endovascular treatment of tiny intracranial aneurysm with difficult geometry. The endovascular coiling of very small ruptured aneurysms with difficult geometry presents a significant treatment challenge because of potential dangerous complications, such as intraprocedural ruptures. We report our initial experience with the...

Oberhuber, A.; Thiere, M.; Simon, F.; Kramer, M.; Einsiedel, T.; Orend, K-H.; Sunder-Plassmann, L.; Schelzig, H., 2012: Endovascular treatment of traumatic ruptures of the thoracic aorta. Traumatic rupture of the descending aorta is an acute life-threatening event. The most common cause is deceleration trauma resulting in a sudden stretching of the aortic isthmus as for example in car and motorcycle accidents and falls from a great...

Sparks, D., 1975: Nutrient concentration and content of pecan fruit. Annual report 6th 33-36

Gunnelius, L.; Hakkila, K.; Kurkela, J.; Wada, H.; Tyystjärvi, E.; Tyystjärvi, T., 2014: The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria. The eubacterial RNA polymerase core, a transcription machinery performing DNA-dependent RNA polymerization, consists of two α subunits and β, β' and ω subunits. An additional σ subunit is recruited for promoter recognition and transcript...

Mohr, J.A.; Pederson, J.A.; Long, H., 1982: Leukocyte survival in hemodialysis patients. Journal of the Oklahoma State Medical Association 75(5): 114-117

Perez Arias J.; Guerrero Lopez F.; Gasco Montes J.M., 1988: Structural stability as an indicator of cement effect on the physical properties of soils. Pages 502-508 1988