geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Manipulation of unfolding-induced protein aggregation by peptides selected for aggregate-binding ability through phage display library screening


, : Manipulation of unfolding-induced protein aggregation by peptides selected for aggregate-binding ability through phage display library screening. Biochemical and Biophysical Research Communications 291(4): 903-907

A phage-displayed library of peptides (12-mer) was screened for the ability to bind to thermally aggregated bovine carbonic anhydrase (BCA), with a view toward examining whether peptides possessing this ability might bind to partially structured intermediates on the protein's unfolding pathway and, therefore, constitute useful tools for manipulation of the kinetic partitioning of molecules between the unfolded and aggregated states. Two peptides (N-HPST-MGLRTMHP-C and N-TPSAWKTALVKA-C) were identified and tested. While neither showed thermal aggregation autonomously, both peptides individually elicited remarkable increases in the levels of thermal aggregation of BCA. A possible explanation is that both peptides bind to surfaces on molten BCA that are not directly involved in aggregation. Such binding could slow down interconversions between folded and unfolded states and states and stabilize aggregation-prone intermediate(s) to make them more prone to aggregation, while failing to achieve any steric prevention of aggregation. The approach has the potential of yielding useful aggregation-aiding/inhibiting agents, and may provide clues to whether amorphous aggregates are "immobilized" forms of folding intermediates.

US$19.90

PMID: 11866450

DOI: 10.1006/bbrc.2002.6549


Other references

Marcus, V.A.; Viloria, J.; Owen, D.; Tsao, M.S., 1996: Malignant rhabdoid tumor of the colon. Report of a case with molecular analysis. Malignant rhabdoid tumors were first described in the kidney as a rare variant of Wilms' tumor with a "rhabdomyosarcomatoid" pattern and a particularly poor prognosis. Further studies have demonstrated these neoplasms as a distinct...

Diepgen, T.L.; Fartasch, M.; Ring, J.; Scheewe, S.; Staab, D.; Szcepanski, R.; Werfel, T.; Wahn, U.; Gieler, U., 2003: Education programs on atopic eczema. Design and first results of the German Randomized Intervention Multicenter Study. Atopic eczema (AE) is a common, chronically relapsing, inflammatory skin disease with an early onset during infancy associated with a high loss of quality of life and socioeconomic burden. In the past few years, an Atopic Eczema Prevention Program...

Chen, S-Jung.; Huang, S-Shyang.; Chang, N-Shan., 2013: Role of WWOX and NF-κB in lung cancer progression. It is generally agreed that the pro-inflammatory, pro-survival transcription factor NF-κB is a tumor promoter. Tumor necrosis factor alpha (TNF-α or TNF) mediates NF-κB activation. Tumor suppressor WWOX (FOR or WOX1) is a downstream effector of...

Parry, Roberta, 2001: EPAs vision The next steps. Journal of Dairy Science 84(Supplement 1): 3

Kleine, M.W., 1997: Introduction to oral enzyme therapy. Hydrolytic enzymes are used in medicine by oral application in various indications. Their intestinal absorption has been proven. They act as 'biological response modifiers' by using different mechanisms, especially by modulating the immu...

V.V.Z.anovich; V.Y. Pushkar’; M.Z.K.lekhsaev, 2011: Specific features of growth and energetics of juvenile rainbow trout Parasalmo Oncorhynchus mykiss at constant temperature and its short-time periodic deviations into the upper suboptimal zone. It was shown that short-time periodical temperature fluctuations within the upper temperature zone suboptimal for the growth of juvenile rainbow trout <i>Parasalmo</i> (<i>Oncorhynchus</i>) <i>mykiss</i> exerts...

Mokeeva, T.M.; Gladkina, T.S.; Ivanov, OA.; Ipat'eva, N.V.M.ier, M.N.; Cheptsova, N.Y., 1964: Mouselike rodents. Unknown

van den Wildenberg, F.A., 1982: The retrocaecal appendix: a snake in the grass. Netherlands Journal of Surgery 34(3): 133-135

Nieves Aldrey, JL., 1984: On some new or interesting cynipid gall-makers (Hym., Cynipidae) from Spain. International Congress of Entomology Proceedings, 17: 912

Cadavid, R.; Jean, B.; Wüstenberg, D., 2009: On the selection of the nozzle geometry and other parameters for cutting corneal flaps with waterjets. A cutting waterjet to produce corneal flaps during refractive surgery or to slice donor corneas for corneal grafting was developed. Jets generated with several different nozzles were compared to determine the most appropriate nozzle geometry for t...