geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Markers of apoptosis in cardiovascular tissues: focus on Annexin V


, : Markers of apoptosis in cardiovascular tissues: focus on Annexin V. Cardiovascular Research 45(3): 549-559

In the last decade, apoptosis (or programmed cell death) has become appreciated as an important process in the development of the cardiovascular system. Moreover, apoptosis contributes to the adaptation of the system to the environment. We are at the beginning of understanding its relevance to cardiovascular physiology and pathology. This understanding forms the key to implement apoptosis in diagnosis and therapy of cardiovascular diseases. New avenues for pharmacological intervention are expected to arise from the synergy of our knowledge about the molecular mechanisms of apoptosis, and how apoptosis integrates in the complex environment of the cardiovascular tissue. The latter strongly depends on techniques to measure apoptosis. Currently, we are facing a relative paucity in available techniques, covering both specificity and sensitivity, and furthermore allowing quantitative analysis, preferably in combination with morphology. This field, however, is rapidly evolving and is fed by the expanding knowledge about the molecular mechanisms of apoptosis. In this paper we will briefly review the available techniques to detect and/or quantify apoptosis. These methods are based on the analysis of cellular morphology, either by light- or electron microscopy, DNA fragmentation (TdT-mediated X-dUTP nick end labeling or in situ nick end labeling), or cytoplasmic and membrane changes. Furthermore, the advantages and limitations of these techniques for their use in cardiovascular research will be outlined. In the text we will refer to available reviews and protocols which discuss the techniques in more detail. The main part of this article will, however, focus on a recently introduced technique, the Annexin V-based apoptosis detection assay. The principle, characteristics, pro's and contra's of this new apoptosis detection assay will be discussed.

US$19.90

PMID: 10728376

DOI: 10.1016/s0008-6363(99)00396-x


Other references

Mattern, C.F.; Daniel, W.A.; Honigberg, B.M., 1969: Structure of Hypotrichomonas acosta (Moskowitz) (Monocercomonadidae, Trichomonadida) as revealed by electron microscopy. Journal of Protozoology 16(4): 668-685

Graziadei, P.P.; Monti Graziadei, G.A., 1985: Neurogenesis and plasticity of the olfactory sensory neurons. Annals of the New York Academy of Sciences 457: 127-142

Schachter, J.; McCormack, W.M.; Chernesky, M.A.; Martin, D.H.; Van Der Pol, B.; Rice, P.A.; Hook, E.W.; Stamm, W.E.; Quinn, T.C.; Chow, J.M., 2003: Vaginal swabs are appropriate specimens for diagnosis of genital tract infection with Chlamydia trachomatis. Because self-collected vaginal swabs (VS) are potentially very useful for screening asymptomatic women for Chlamydia trachomatis infection, a multicenter study evaluated that specimen with nucleic acid amplification tests (NAATs). The objective wa...

Geluso K.N.; Studier E.H., 1979: Diurnal fluctuation in urine concentration in the little brown bat myotis lucifugus in a natural roost. Urine collected periodically from naturally roosting M. lucifugus on a rainy, overcast day and on a successive sunny, warm day showed no differences in concentration between sexes, age groups, or activity level (torpid or active). Time of day at w...

Reddy, M.S.S.inivasa; Trivedi, P.K.; Tuli, R.; Sane, P.V., 1997: Expression of chloroplastic genes during autumnal senescence in a deciduous tree Populus deltiodes. In Populus deltoides, a deciduous tree, the development on new leaves starts in the month of March, the leaves reach maturity by October and fall by December. Changes in the composition and function of the photosynthetic apparatus were analysed du...

Choi, C-Young.; Han, S-Rok.; Yee, G-Taek.; Lee, C-Heuck., 2012: Spontaneous regression of an unruptured and non-giant intracranial aneurysm. It is well known that spontaneous thrombosis in giant cerebral aneurysm is common. However, spontaneous obliteration of a non-giant and unruptured cerebral aneurysm has been reported to be rare and its pathogenic mechanism is not clear. We describ...

Abraham, W.T., 2007: Intrathoracic impedance monitoring for early detection of impending heart failure decompensation. In patients with chronic heart failure, detecting signs and symptoms of worsening congestion early enough to prevent hospitalization is an ongoing challenge. Intrathoracic impedance monitoring is a new, device-based method for detecting fluid accu...

O'Riordan, B.G.; Daniel, F.J.; Fletcher, D.R., 1992: Open lung biopsy without thoracotomy: technique and possible indications. Lung biopsy material is still required in the diagnosis of lung disease, especially in diffuse parenchymal diseases or pulmonary infiltrates in the immunosuppressed patient. This often requires open thoracotomy which is not without morbidity or mo...

Medvecky, D., 1983: Research on the effect of different daily frequency and technique of milk feeding of calves on their yield. In 3 trials 95 calves were given milk replacers Kolostran and Laktavit, feed concentrate TK-S, hay, and maize silage. Milk replacer was given in pails to calves once or twice a day from 11, 17 or 25 days old or from an automatic feeder, for 90 day...

Bloesch, M., 1980: Drei Jahrzehnte Schweizerischer Storchansiedlungsversuch (Ciconia ciconia) in Altreu, 1948-1979. Erfuhrung bei Haltung, Aufzucht und Ansiedlung der Versuchsstorche. Ornithologische Beobachter, 773: 167-194