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Mast cells express connexins on their cytoplasmic membrane


, : Mast cells express connexins on their cytoplasmic membrane. Journal of Allergy & Clinical Immunology 103(4): 656-662, April

Background: Because of the close association between mast cells and fibroblasts in the microenvironment and the importance of connexins (Cxs) in fibroblast communication with other cells, we hypothesized that mast cells also express Cxs, allowing them to similarly communicate with other cells through gap junctions. Objectives: We sought to identify the expression of Cxs (particularly Cx43, Cx32, and Cx26) by murine mast cells. Methods: The expression of Cxs was studied by RT-PCR, Northern blot analysis, Western blot analysis, flow cytometry, and confocal laser scanning microscopy. Results: In this report we demonstrate that murine bone marrow cultured mast cells and the growth factor-independent murine mast cell line C57, express Cx43 and Cx32 as assessed by RT-PCR, Northern blot analysis, Western blot analysis, and flow cytometry, but do not express Cx26. We also show, by confocal laser scanning microscopy, that Cx43 localizes to the cytoplasmic membrane of mast cells in a pattern similar to that seen in fibroblasts. Conclusions: Mast cells express Cx43 and Cx32, and Cx43 is associated with the cytoplasmic membrane, suggesting that mast cells have the potential to communicate with other cells in their microenvironment in part through gap junctions.

US$19.90

PMID: 10200016

DOI: 10.1016/s0091-6749(99)70239-3


Other references

Meszaros, M.; Morton, D.B., 1996: Expression of a developmentally regulated gene, mng10, in identified neurosecretory cells in the CNS of Manduca sexta. We are interested in the molecular events underlying the development of the nervous system of Manduca sexta during the final 24 h of the pupal molt. In this article we describe a gene, Mng10, that is expressed in the abdominal nervous system of M....

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Citron, R., 1994: Doubling time of cancer (junk science in the courtroom). Legal Medicine: 1-56

Peter R.Shaw, 1987: Investigations of relative plate motions in the South Atlantic using Seasat altimeter data. Journal of Geophysical Research: Solid Earth 92(B9): 9363-9375

Velasco, M.; Velasco, F.; Cepeda, C.; Estrada-Villanueva, F., 1977: Alumina cream induced focal motor epilepsy in cats. II. Wakefulness--sleep modulation of pyramidal tract multiple unit activity. Electroencephalography and Clinical Neurophysiology 43(1): 67-73

Schlack, C.A., 1944: Rate of occurrence of epithelial tissue in periapical lesions. Of 103 periapical lesions, which were sectioned and examined, 42% contained epithelial tissue in different stages of differentiation. The author emphasized that following tooth extraction, all periapical lesions should be removed.

Kurien, B.T.; Scofield, R.Hal., 2002: Extraction of nucleic acid fragments from gels. A major complication of autologous stem cell transplantation (ASCT) is the development of therapy-related myelodysplastic syndromes (MDS) and acute myelogenous leukemia (AML). This complication likely results from previous exposure of the autologo...

Ellison, S.P.; Jr., 1964: Second supplement to the annotated bibliography and index of conodonts. Texas Journal of Science, 16: 216-242

Li, Q.; Skyberg, J.A.; Fakhr, M.K.; Sherwood, J.S.; Nolan, L.K.; Logue, C.M., 2006: Antimicrobial susceptibility and characterization of Salmonella isolates from processed bison carcasses. Seventeen Salmonella enterica serovar Hadar isolates recovered from bison were found to possess a range of virulence genes and resistance to tetracycline, gentamicin, sulfamethoxazole, and streptomycin simultaneously. A 1-kb class 1 integron conta...

Wang Y.M.i; X.Y.n Yuan; Jia Jing Fen, 2000: Transformations system and study on the introduction of chitinase gene into Linum usitatissium. Optimal shoot differentiation from hypocotyl explants of Linum usitatissium was MS medium supplemented with BA 1 mg/L and IAA 0. 5 mg/L, which resulted in 97% of shoot regeneration frequency. The hypocotyl explants were infected by Agrobacterium t...