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Mechanical stress induces COX-2 mRNA expression in bone cells from elderly women

, : Mechanical stress induces COX-2 mRNA expression in bone cells from elderly women. Journal of Biomechanics 33(1): 53-61, Jan

Mechanical loading-induced fluid flow in the lacuno-canalicular network is a possible signal for bone cell adaptive responses. In an earlier study we found that pulsating fluid flow (PFF, 0.7 mu 0.02 Pa, 5 Hz, 0.4 Pa/s) stimulates the production of prostaglandins by neonatal mouse calvarial cells. In addition, mRNA expression of the inducible form of cyclooxygenase (COX-2), but not the constitutive form (COX-1), the major enzymes in prostaglandin production, was increased by PFF. The present study was performed to determine whether human primary bone cells from the iliac crest, respond to mechanical stress in a similar way as neonatal mouse calvarial cells. We subjected bone cells originating from the iliac crest of nine elderly women, between 56 and 80 yr of age, for 1 h to PFF and measured prostaglandin production and COX-1 and COX-2 mRNA expression. One hour PFF treatment stimulated the release of PGE2 by 3.5 fold and PGI2 by 2.2 fold. PFF also increased the expression of COX-2 mRNA by 2.9 fold, but did not change COX-1 mRNA. No correlation was found between donor age and PFF effect, neither on prostaglandin production nor on COX-2 mRNA expression. This study shows that bone cells from the iliac crest of elderly women react to PFF treatment in a similar way as neonatal mouse calvarial cells, namely with increased production of prostaglandins and upregulation of COX-2 mRNA expression. These results suggest that human bone cells from the iliac crest and neonatal mouse calvarial cells share a similar mechanotransduction pathway.


DOI: 10.1016/s0021-9290(99)00172-4

Other references

Keen, W.W., 1903: The Duties And Responsibilities Of Trustees Of Public Medical Institutions. Science 17(438): 801-812

Mihăilescu, A., 1980: Evaluation of development of children stimulated by organized games. Viata Medicala; Revista de Informare Profesionala Si Stiintifica A Cadrelor Medii Sanitare 28(9): 199-201

Nagasao, T.; Nakajima, T.; Kimura, A.; Kaneko, T.; Jin, H.; Tamaki, T., 2005: The dynamic role of buttress reconstruction after maxillectomy. The purpose of this study was to investigate the dynamic effect of maxillary reconstruction after partial resection of the maxilla. On a personal computer, three-dimensional maxilla models were designed based on computed tomographic data obtained...

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Wolfe, R.I.; Irvine, R.B.; Campbell, K.W., 1996: Registration of 'Leduc' barley. Registered in Canada in 1982, this 6-rowed spring feed barley variety, derived from the cross Steptoe/Klondike and widely adapted across the Canadian prairies, has been described previously [Canadian Journal of Plant Sciences (1988) 68 (1) 221-223...

Anonymous, 1967: Granulomatous colitis. New England Journal of Medicine 277(6): 310-311

Bruhn, D.; Mikkelsen, T.N.; Atkin, O.K., 2002: Does the direct effect of atmospheric CO2 concentration on leaf respiration vary with temperature? Responses in two species of Plantago that differ in relative growth rate. The objective of this study was to investigate the direct effect of elevated atmospheric CO2 concentrations on leaf respiration in darkness (R) over a broad range of measurement temperatures. Our aim was to further elucidate the underlying mechani...

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Rose, E.K.; Shorey, W.K., 1977: Memoir of Winston Kinney Shorey (1919-76). Transactions & Studies of the College of Physicians of Philadelphia 44(4): 208-209

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