geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Mechanism of TCDD-induced suppression of antibody production: effect on T cell-derived cytokine production in the primary immune reaction of mice


, : Mechanism of TCDD-induced suppression of antibody production: effect on T cell-derived cytokine production in the primary immune reaction of mice. Toxicological Sciences 70(1): 46-54

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is known to suppress antigen-specific antibody production in humoral immune reactions, but the precise mechanism remains unclear. Since T cell activation and subsequent production of type 2 helper T (Th2) cell-derived cytokines are required for antigen-specific antibody production in humoral immunity, we examined the effects of TCDD on splenic T cell numbers, T cell growth factor IL-2 production, and Th2 cell-derived cytokine production. C57BL/6N mice were orally given TCDD (20 microg/kg) or vehicle, and immediately intraperitoneally immunized with ovalbumin (OVA) adsorbed to alum, and cellular changes in the spleen and cytokine production by spleen cells were investigated from Day 1 to Day 14. In vehicle-control mice the numbers of splenic CD3(+) T cells increased from Day 7 onward, but no increase was observed in the TCDD-exposed mice. When spleen cells from control mice were cultured and restimulated with OVA ex vivo, a significant amount of IL-2 was found from Day 1, but it decreased on Day 7, whereas TCDD exposure promptly suppressed the increase on Day 4. TCDD exposure significantly suppressed the production of Th2 cell-derived cytokines IL-4, IL-5, and IL-6, which were prominently increased from Day 4 onward in control mice. The dose-dependent study showed that IL-5 production was significantly suppressed in a dose-dependent manner starting at 1 microg/kg TCDD. Moreover, separation and reconstitution studies showed that the TCDD-induced suppression of IL-5 production was due to the impaired function of T cells rather than that of antigen-presenting cells. The results of this study suggest that TCDD-induced suppression of T cell activation and Th2-type cytokine production is involved in the impairment of antigen-specific antibody production.

US$19.90

PMID: 12388834

DOI: 10.1093/toxsci/70.1.46


Other references

Islam, M.Tofazzal.; von Tiedemann, A., 2012: 2,4-Diacetylphloroglucinol suppresses zoosporogenesis and impairs motility of Peronosporomycete zoospores. 2,4-Diacetylphloroglucinol (DAPG) produced by Pseudomonas fluorescens, shows toxicity to many microorganisms including fungi, bacteria, and peronosporomycetes. Zoosporogenesis and motility of zoospores are critical for a complete disease cycle and...

Mueller I., 1982: Hydrogeology of the Areuse source drainage basin, Neuchatel Jura, excursion E of April 16, 1982. Jahresberichte und Mitteilungen des Oberrheinischen Geologischen Vereines: ages 61-72. 1982.

Mutsaers, S.E.; D.P.olo, N., 2007: Future directions in mesothelial transplantation research. International Journal of Artificial Organs 30(6): 557-561

Coffey, J.D.; Honaker, E., 1949: Acute infectious lymphocytosis; a case report. Mississippi Doctor 26(10): 278-278

Yadav, R.P.; Agrawal, C.S.; Gupta, R.K.; Rajbansi, S.; Bajracharya, A.; Adhikary, S., 2010: Perforated duodenal ulcer in a young child: an uncommon condition. Duodenal ulcer is an uncommonly diagnosed entity in children. H. pylori infection, blood group 'O' or secondary to medications like non steroidal anti-inflammatory drugs (NSAID) and corticosteroids or physiological stress in burns, head...

Klopfer F.D.; Kilgour R.; Matthews L.R., 1981: Paired comparison analysis of palatabilities of 20 foods to dairy cows. Proceedings of the New Zealand Society of Animal Production 41: 242-247

Rokosová-Cmuchalová, B.; Bentley, J.P., 1968: Relation of collagen synthesis to chondroitin sulfate synthesis in cartilage. The question of whether the formation of mucopolysaccharide and collagen are mutually dependent processes was studied in an in vitro system. Puppy epiphyseal cartilage was incubated in the presence of sodium salicylate or [alpha],[alpha][image]-di...

Miller, Tr, 1973: Supervised occupational experience: modern and comprehensive. Agricultural education: 45 (11) 254

L.Moult, E., 1931: Formes nouvelles ou peu connues de Morpho (Lep.). Ire note. Novitates Entomologicae, 1: 5-8

Kamii, I., 1962: Electrodialysis of organic acids, organic bases, and alkaloids across ion exchange membranes. III. On the permeability of caffeine and ephedrine. Yakugaku Zasshi 82: 903-906