geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Mobile foci of Sp100 do not contain PML: PML bodies are immobile but PML and Sp100 proteins are not


, : Mobile foci of Sp100 do not contain PML: PML bodies are immobile but PML and Sp100 proteins are not. Journal of Structural Biology 140(1-3): 180-188, Oct -Dec

PML bodies are nuclear organelles that are associated with various diseases and are suggested to be involved in multiple cellular activities including transcriptional regulation, apoptosis, and antiviral defence. Because many proteins with different functions aggregate in PML bodies, it has also been suggested that these bodies function as nuclear depots. Some proteins consistently found in PML bodies may form a stable scaffold that regulates the recruitment of other proteins. Thus, some proteins might be stably integrated into PML bodies while others continuously exchange with the nucleoplasm. To study the dynamic properties of PML bodies and resident proteins, we constructed fusion proteins of Sp100, PML, and CBP with autofluorescent proteins. Using time-lapse imaging, we show that PML bodies exhibit little movement but that small foci that contain Sp100 but not PML are dynamic and fuse with PML bodies. Furthermore, we show by monitoring fluorescence recovery after photobleaching that Sp100, PML, and CBP are dynamic components of PML bodies. This suggests that these proteins do not play a strict structural role in these bodies but that they function at other sites in the nucleoplasm.

US$19.90

PMID: 12490166

DOI: 10.1016/s1047-8477(02)00529-4


Other references

Drachev, L.A.; Kaulen, A.D.; Khitrina, L.V.; Skulachev, V.P., 1981: Fast stages of photo electric processes in biological membranes 1. bacterio rhod opsin. Bacteriorhodopsin-containing fragments of Halobacterium halobium membrane (bacteriorhodopsin sheets) were incorporated into a lecithin-impregnated collodion film, and fast stages of flash-induced electrogenesis were measured by 2 electrodes separa...

Palma-Dibb, R.Guenka.; Taba, Mário.; Santos, C.Moreto.; Navarro, Véria.Pontelli., 2004: Autogenous tooth fragment reattachment--association of periodontal surgery and endodontic and restorative procedures: a case report. The occurrence of dental traumas in adult patients is on the rise, according to the literature, and require correct and adequate treatment specific to each fracture in order to preserve the remaining tooth. In this article, two different fracture...

Desmyter, J.; Melnick, J.L.; Rawls, W.E., 1968: Defectiveness of interferon production and of rubella virus interference in a line of African green monkey kidney cells (Vero). Vero cells, a line of African green monkey kidney cells, failed to produce interferon when infected with Newcastle disease, Sendai, Sindbis, and rubella viruses, although the cells were sensitive to interferon. Further, infection of Vero cells wit...

Jones, K.R.; Pillsbury, H.C., 1990: Cellulose fiber diet pills. A new cause of esophageal obstruction. Cellulose fiber diet pills have recently become a popular form of weight control. In the past 2 months, we have seen two patients in whom ingestion of these pills has resulted in complete distal esophageal obstruction. Further studies revealed tha...

Nisengard R.J.; Myers D.; Fischman S.; Socransky S., 1976: Immunologic studies of acute necrotizing ulcerative gingivitis. Journal of Dental Research 55(SPEC ISSUE B): B206

Long B.; Schillinger S.; Soria J., 1998: Genesis and architecture of an intertidal sand spit; the Banc du Bucheron, Re Island, France. Pages 513 1998

Obrecht, P.; Woenckhaus, J.W.; Strickstrock, K.H., 1964: On The Demonstration Of N-Methyl-Bis-Chloroethylamine (Dichloren) In The Body Of Rats With 4-P-Nitrobenzylpyridine (Nbp). Zeitschrift für Krebsforschung 66: 151-154

Gernert, E.R., 1956: The use of antibiotics in the treatment of pulmonary tuberculosis. Journal of the Kentucky State Medical Association 54(4): 347-351

Sacco, S., 1974: The use of antibiotics and antiphlogistics in the therapy of periodontitis. Rivista Italiana di Stomatologia 29(10-12): 427-434

Dice, Lee R., 1939: The Sonoran biotic province. The Sonoran biotic province is distinguished by its assemblage of species and races of plants and of animals. Many of the peculiar Sonoran races and species of amphibians, reptiles, and mammals have probably differentiated in the province, due evi...