geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Modulation of prostacyclin and thromboxane secretion by cytotrophoblasts from normal and pre-eclamptic human pregnancies


, : Modulation of prostacyclin and thromboxane secretion by cytotrophoblasts from normal and pre-eclamptic human pregnancies. Placenta 23(8-9): 594-599, September-October

We and others have previously observed an imbalance in cytotrophoblast secretion of the vasoactive prostanoids prostacyclin and thromboxane A2 in pre-eclampsia. To examine the effects of potential modulators of this imbalance, cytotrophoblasts isolated from normal and pre-eclamptic pregnancies were incubated in the presence of lipopolysaccharide, the calcium ionophore A23187, tumour necrosis factor alpha, or interleukin 1beta, with or without the cyclo-oxygenase inhibitor, indomethacin. Further incubations included the drugs tranylcypromine, a prostacyclin synthetase inhibitor (0.1, 10 muM), or the thromboxane synthetase inhibitor, pirmagrel (0.001, 1 muM). Results showed that cytotrophoblasts from pre-eclamptic pregnancies had increased thromboxane production and significant stimulation of prostacyclin production by lipopolysaccharide and calcium ionophore. Lipopolysaccharide stimulated thromboxane production in normal cytotrophoblasts, while indomethacin almost completely inhibited production of both prostanoids. Tranylcypromine mildly inhibited prostacyclin production in normal cytotrophoblasts only, whereas pirmagrel strongly inhibited thromboxane production in a dose-related manner, with reciprocal increase in prostacyclin production occurring in cytotrophoblasts from pre-eclamptic pregnancies. This study confirmed that cytotrophoblasts from pre-eclamptic women had increased thromboxane production and showed that pirmagrel, at the relatively low dose of 0.001 muM, was able to normalize the imbalance of thromboxane and prostacylin production and may therefore warrant further investigation as a treatment for pre-eclampsia.

US$19.90

PMID: 12361679

DOI: 10.1053/plac.2002.0851


Other references

Fox T.; Madsen J.; Van Rhijn J., 1991: Second international symposium on western palearctic geese kleve germany february 7 12 1989. Ardea 79(2): 113-370

Sasaki, T.; Masubuchi, A.; Yamamura, M.; Watanabe, N.; Hiratsuka, M.; Mizugaki, M.; Itoh, K.; Tanaka, Y., 2006: Rat strain differences in stereospecific 2-oxidation of RS-8359, a reversible and selective MAO-A inhibitor, by aldehyde oxidase. Aldehyde oxidase catalysed 2-oxidation activity of the (S)-enantiomer of RS-8359, a selective and reversible monoamine oxidase A (MAO-A) inhibitor, was investigated in liver cytosolic fractions from ten rat strains. Remarkably large strain differe...

Kushner J.P.; Steinmuller D.P.; Lee G.R., 1973: Inhibition of uro porphyrinogen decarboxylase activity by iron. Clinical Research 21(2): 266

Linner, J.H., 1987: Overview of surgical techniques for the treatment of morbid obesity. A brief review of current and antecedent surgical techniques is presented with advantages and disadvantages of the most common operations.

Kang, H.; Kim, S.Y.; Park, W.J., 2001: An improved strategy for a genetic assay for site-specific proteolysis. We have previously reported a genetic assay that is suitable for the study of substrate specificity of a protease in vivo, and herein present a simplified version of the method. In this procedure, expressed in Saccharomyces cerevisiae by using the...

Kaburakis, A., 2007: International prospective student-athletes and NCAA Division I amateurism. This paper summarises a research stream dealing with the US system of amateur intercollegiate sport and its relation to international prospective student-athletes (IPSAs). Crucial differences exist between the US 'clear demarcation' of c...

Qamar, A.; Veeranna, V.; Fatima, A.; Alexander, K.M.; D.C.rli, M.F.; Bonaca, M.P.; Steigner, M.; Dorbala, S., 2016: Utility of multimodality imaging in myopericarditis with aortitis. Journal of Nuclear Cardiology

Moss, B.L.; Magleby, K.L., 2001: Gating and conductance properties of BK channels are modulated by the S9-S10 tail domain of the a subunit. A study of mSlo1 and mSlo3 wild-type and chimeric channels. The COOH-terminal S9-S10 tail domain of large conductance Ca2+-activated K+ (BK) channels is a major determinant of Ca2+ sensitivity (Schreiber, M., A. Wei, A. Yuan, J. Gaut, M. Saito, and L. Salkoff. 1999. Nat. Neurosci. 2:416-421). To investigat...

Manser, T.; Tumas-Brundage, K.M.; Casson, L.P.; Giusti, A.M.; Hande, S.; Notidis, E.; Vora, K.A., 1998: The roles of antibody variable region hypermutation and selection in the development of the memory B-cell compartment. Somatic hypermutation and selection of immunoglobulin (Ig) variable (V)-region genes, working in concert, appear to be essential for memory B-cell development in mammals. There has been substantial progress on the nature of the cis-acting DNA elem...

Ashfaq, R.; Saboorian, M.H.ssein, 1994: Radiologically assisted fine needle aspiration and core biopsies A comparison. Acta Cytologica 38(5): 797