geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Mutations in multidrug efflux homologs, sugar isomerases, and antimicrobial biosynthesis genes differentially elevate activity of the sigma(X) and sigma(W) factors in Bacillus subtilis


, : Mutations in multidrug efflux homologs, sugar isomerases, and antimicrobial biosynthesis genes differentially elevate activity of the sigma(X) and sigma(W) factors in Bacillus subtilis. Journal of Bacteriology 182(18): 5202-5210

The sigma(X) and sigma(W) extracytoplasmic function sigma factors regulate more than 40 genes in Bacillus subtilis. sigma(W) activates genes which function in detoxification and the production of antimicrobial compounds, while sigma(X) activates functions that modify the cell envelope. Transposon mutagenesis was used to identify loci which negatively regulate sigma(W) or sigma(X) as judged by up-regulation from the autoregulatory promoter site P(W) or P(X). Fourteen insertions that activate P(W) were identified. The largest class of insertions are likely to affect transport. These include insertions in genes encoding two multidrug efflux protein homologs (yqgE and yulE), a component of the oligopeptide uptake system (oppA), and two transmembrane proteins with weak similarity to transporters (yhdP and yueF). Expression from P(W) is also elevated as a result of inactivation of at least one member of the sigma(W) regulon (ysdB), an ArsR homolog (yvbA), a predicted rhamnose isomerase (yulE), and a gene (pksR) implicated in synthesis of difficidin, a polyketide antibiotic. In a parallel screen, we identified seven insertions that up-regulate P(X). Remarkably, these insertions were in functionally similar genes, including a multidrug efflux homolog (yitG), a mannose-6-phosphate isomerase gene (yjdE), and loci involved in antibiotic synthesis (srfAB and possibly yogA and yngK). Significantly, most insertions that activate P(W) have little or no effect on P(X), and conversely, insertions that activate P(X) have no effect on P(W). This suggests that these two regulons respond to distinct sets of molecular signals which may include toxic molecules which are exported, cell density signals, and antimicrobial compounds.

US$29.90

PMID: 10960106


Other references

Taylor, J.H.; French, J.A., 2016: Oxytocin and vasopressin enhance responsiveness to infant stimuli in adult marmosets. The neuropeptides oxytocin (OT) and arginine-vasopressin (AVP) have been implicated in modulating sex-specific responses to offspring in a variety of uniparental and biparental rodent species. Despite the large body of research in rodents, the eff...

Vivas, C.A.; Gritsch, H.A.; Shapiro, R.; Scantlebury, V.P.; Hickey, D.P.; Hakala, T.R.; Jordan, M.L., 1997: Cadaveric renal transplantation using donors over 60 years old--5 year follow-up. Transplantation Proceedings 29(1-2): 112-112

Caro, M.L.; Estes, D.M.; Cohn, L.A.; Narfstrom, K., 2002: The Systemic and Ocular Immune Response After Recombinant Adeno-Associated Virus Gene Transfer of RPE65 in 6 Dogs Affected With the RPE65 Null Mutation. Purpose: To evaluate potential systemic and local adverse effects after recombinant Adeno-Associated Virus (rAAV) gene transfer of Retinal Pigmented Epithelium 65 (RPE65) into the subretinal space of dogs affected with the RPE65 null mutation. Met...

Soybir, G.; Köksoy, F.; Ekiz, F.; Yalçin, O.; Ozşeker, A.; Cokneşeli, B., 1998: Effect of mangan-desferrioxamin in the prevention of peritoneal adhesions. An experimental study was carried out using mangan-desferrioxamin chelate, an effective iron-chelating and free oxygen radical scavenging agent, to prevent post-operative peritoneal adhesions in 40 Wistar albino rats. The density of adhesions was...

Kocerba-Soroka, W.; Fiałkowska, E.; Pajdak-Stós, A.; Klimek, B.; Kowalska, E.; Drzewicki, A.; Salvadó, H.; Fyda, J., 2013: The use of rotifers for limiting filamentous bacteria Type 021N, a bacteria causing activated sludge bulking. The excessive growth of filamentous bacteria and the resultant bulking of activated sludge constitute a serious problem in numerous wastewater treatment plants. Lecane inermis rotifers were previously shown to be capable of reducing the abundance...

Alexander, G.L., 2008: A state profile of IT sophistication in nursing homes. In healthcare, IT Sophistication has been defined as the diversity and maturity of information system hardware and software that support clinical services. Clinical services relevant to this study include resident care management, clinical support...

Krummel-McCracken, K., 2011: Stroke as a complication of H1N1 influenza infection: a case study. In June 2010, the Centers for Disease Control and Prevention ended the US public health emergency for the H1N1 pandemic with estimates for total cases, hospitalizations, and deaths counted from April 2009 to April 2010. By the end of this period,...

Holt J.E.; Schoorl D., 1981: Fruit packaging and handling distribution systems an evaluation method. Fruit and vegetable distribution systems vary in efficiency according to the inherent characteristics of the produce and the packaging and the handling procedures adopted. The maintenance of quality depends on the interrelationships of these compo...

Zheng, X.E.tahir, E., 1998: The role of vegetation in the dynamics of West African monsoons. The focus of this paper is the role of meridional distribution of vegetation in the dynamics of monsoons and rainfall over West Africa. A moist zonally symmetric atmospheric model coupled with a simple land surface scheme is developed to investiga...

Steinen Randolph P.; Harrison Randolph S.; Matthews R.K., 1973: Eustatic Low Stand of Sea Level between 125,000 and 105,000 B.P.; evidence from the Subsurface of Barbados, West Indies. Brown micritic crust at 32 m below sea level (borehole), in fore-reef skeletal wackestone, subaerial, overlying quart-bearing grainstone containing clasts of the crust, intense glacial event.