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On-line extraction-spectrophotometric determination of neostigmine in pharmaceuticals using double membrane phase separator and monovalent dyestuff

, : On-line extraction-spectrophotometric determination of neostigmine in pharmaceuticals using double membrane phase separator and monovalent dyestuff. Talanta 49(5): 997-1001, Aug 9

A simple, sensitive and rapid spectrophotometric method for the determination of neostigmine by flow injection analysis (FIA) coupled with an ion associate extraction has been developed. The three-line manifold was assembled. Neostigmine(200 mul) was injected into a distilled water stream and the pH was adjusted to 10 with a borate-phosphate buffer solution. Then, the stream was mixed with the ion-pairing tetrabromophenolphthalein ethylester (TBPEH)-1,2-dichloroethane solution. After phase separation with a double membrane phase separator, absorbance was measured at 610 nm. A linear calibration graph was obtained between 1 X 10-7 mol l-1 and 5 X 10-7 mol l-1 of neostigmine. Up to 48 samples h-1 could be processed with a relative standard deviation (R.S.D.) of 0.5% (n = 5) for 4 X 10-7 mol l-1 neostigmine. The proposed system was applied to the simple, reproducible and rapid determination of neostigmine in commercial pharmaceuticals.


DOI: 10.1016/s0039-9140(99)00017-x

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