geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ovarian steroid effects on serotonin 1A, 2A and 2C receptor mRNA in macaque hypothalamus


, : Ovarian steroid effects on serotonin 1A, 2A and 2C receptor mRNA in macaque hypothalamus. Molecular Brain Research 63(2): 325-339, Jan 8

This study mapped the location of serotonin (5HT) 1A, 2A and 2C receptor mRNA expression in the female macaque hypothalamus and determined whether the expression was regulated by estrogen plus or minus progesterone treatment using in situ hybridization (ISH) and densitometric analysis of autoradiographic films. The experimental groups of pigtail macaques (Macaca nemestrina) were spayed controls (n = 4), estrogen treated (28 days, n = 4) and estrogen + progesterone-treated animals (14 days estrogen + 14 days estrogen and progesterone, n = 4). Monkey specific 5HT1A (432 bp), 2A (411 bp) and 2C (294 bp) receptor probes were generated with PCR. Moderate 5HT1A receptor hybridization signal was detected in the preoptic area and the ventromedial nuclei. Less intense 5HT1A receptor signal was detected in a contiguous area from the dorsomedial nuclei through the posterior hypothalamus and in the supramammillary area. There was no change in 5HT1A receptor hybridization signal in any area with ovarian steroid treatment. Dense 5HT2A receptor hybridization signal was morphologically confined to the paraventricular, supraoptic, and mammillary nuclei and the external capsule of the thalamus. Light 5HT2A mRNA signal was inconsistently observed in the ventromedial nuclei. There was no change in the 5HT2A receptor hybridization signal in any area with ovarian steroid treatment. The 5HT2C receptor mRNA was widely distributed in the macaque hypothalamus. The preoptic area and anterior hypothalamus were largely positive for 5HT2C mRNA with a more concentrated signal in a narrow periventricular area. Dense 5HT2C receptor signal was detected lateral to the ventromedial nuclei (capsule), in the tuberomammillary nuclei, arcuate nucleus, dorsomedial nuclei, infundibular area and choroid plexus. Moderate 5HT2C receptor signal was detected in the ventromedial nuclei, lateral hypothalamus and dorsal to posterior hypothalamus. There was a significant decrease in total 5HT2C mRNA hybridization signal with ovarian steroid treatment in the ventromedial nuclei, dorsal and posterior hypothalamus. In summary, macaque 5HT1A, 2A and 2C receptor mRNAs are located in distinct hypothalamic loci which play a role in a number of autonomic functions and behavior. Ovarian steroids decreased the expression of 5HT2C receptor mRNA in the ventromedial nuclei, dorsal and posterior hypothalamus. The expression of 5HT1A and 5HT2A receptor mRNA was not altered by treatment with ovarian steroids.

US$19.90

PMID: 9878811

DOI: 10.1016/s0169-328x(98)00295-2


Other references

Wall, J.; Hellman, E.; Denend, L.; Rait, D.; Venook, R.; Lucian, L.; Azagury, D.; Yock, P.G.; Brinton, T.J., 2016: The Impact of Postgraduate Health Technology Innovation Training: Outcomes of the Stanford Biodesign Fellowship. Stanford Biodesign launched its Innovation Fellowship in 2001 as a first-of-its kind postgraduate training experience for teaching biomedical technology innovators a need-driven process for developing medical technologies and delivering them to pa...

Veloz, MA., 1989: Mar, hombre y arte. Unknown

Horschig, A.D.; Neff, T.E.; Serrano, A.J., 2014: Utilization of autoregulatory progressive resistance exercise in transitional rehabilitation periodization of a high school football-player following anterior cruciate ligament reconstruction: a case report. The Autoregulatory Progressive Resistance Exercise (APRE) model of periodization is an effective form of resistance training programming for short-term training cycles in healthy athletic populations that has yet to be effectively described in lit...

Carter, T.D.; Kurent, J.E., 1994: Clinical syndromes of cerebral ischemia. Journal of the South Carolina Medical Association 90(7): 310-313

Dong, Z.; Zhang, L.; Jia, X.; Zhou, J.; Wu, C., 2016: Mixed squamous cell and glandular papilloma: a report of 4 cases and review of literature. Zhonghua Bing Li Xue Za Zhi 44(11): 792-793

Reis, M.V., 1961: Physical education in the rehabilitation of visual disorders. Medicina Contemporanea 79: 231-239

Scheffler, S.; Chwastek, H.; Schönfelder, V.; Unterhauser, F.; Hunt, P.; Weiler, A., 2005: The impact of radiofrequency shrinkage on the mechanical and histologic properties of the elongated anterior cruciate ligament in a sheep model. Purpose: Shrinkage of the elongated anterior cruciate ligament (ACL) and its grafts with radiofrequency (RF) energy has become increasingly popular as an alternative to ACL reconstruction in the field of knee surgery. Although there have been a fe...

Baroncelli, Maria Angela., 2000: First evidence of Parastrophia asturiana De Folin, 1870 Gastropoda, Cecidae from the piedmontese Pliocene NW Italy Prima segnalazione di Parastrophia asturiana De Folin, 1870 Gastropoda, Cecidae nel Pliocene piemontese Italia, NW. Two Parastrophia asturiana De Folin, 1870 specimens from sandy-silty sediments of the Botto Valley, near Asti (Piedmont, NW Italy) are described. Morphologic remarks and distribution of fossil, and actual P. asturiana are given. A synonimic analys...

Oancea, E.; Vriens, J.; Brauchi, S.; Jun, J.; Splawski, I.; Clapham, D.E., 2009: TRPM1 forms ion channels associated with melanin content in melanocytes. TRPM1 (melastatin), which encodes the founding member of the TRPM family of transient receptor potential (TRP) ion channels, was first identified by its reduced expression in a highly metastatic mouse melanoma cell line. Clinically, TRPM1 is used...

Saradeth, T.; Quittan, M.; Ghanem, A.H.; Ernst, E., 1995: Improvement of hemorheological variables induced by phlogenzyme. Biorheology 32(2-3): 323-324