geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli


, : PCR cloning of Pseudomonas resinovorans polyhydroxyalkanoate biosynthesis genes and expression in Escherichia coli. Biotechnology Letters 22(9): 789-794, May

A ca. 5.5-kb region of Pseudomonas resinovorans genome containing the polyhydroxyalkanoate (PHA) biosynthesis locus was sequenced. Three complete open-reading-frames (ORFs), i.e., phaC1Pr, phaZPr, and phaC2Pr, were identified. Using this sequence information, phaC1Pr was PCR-cloned from P. resinovorans genomic DNA and expressed in E. coli as shown by a Nile Red plate assay and gas chromatography/mass spectrometric analysis.

US$19.90

DOI: 10.1023/a:1005614209342


Other references

Kameya T.; Lee H Y.; Toki S.; Kanzaki H., 1992: Induction of transgenic tobacco plants by utilization of fertilization cycle. Pistils of tobacco [Nicotiana tabacum] plants were treated with the DNA of the plasmid pBI121 at various times after pollination. When the treatment was applied at 36 and 48 hours after pollination, transgenic plants were obtained. This observatio...

Warren, R.E.; Warnock, D.W., 1982: Clinical manifestations and management of aspergillosis in the compromised patient. This review includes discussion of the natural habitat of Aspergillus spp.; mode of infection; clinical manifestations of invasive aspergillosis; diagnosis of invasive aspergillosis; choice of treatment; and prevention of infection.

Shutilova, N.I.; Kadoshnikova, I.G.; Kozlovskaia, N.G.; Klevanik, A.V.; Zakrzhevskiĭ, D.A., 1979: Optimization of isolation conditions for three types of pigment protein-lipid complexes from chloroplasts during solubilization with Triton X-100. The maximal total release of pigment protein-lipid complexes (PPLC) during their isolation from pea chloroplasts was achieved by 1-hr solubilization with Triton X-100, the Triton:chlorophyll (T/Chl) ratio being 50 mg/mg/ml. The total yield of the...

Roslyakov, GE., 1985: Falcipennis falcipennis of Khabarovsk Territory is in need of protection. Unknown

Recchioni, R.M., 1969: Use of an anabolizing cream in the healing process of the simple cervix erosion (personal study). Minerva Ginecologica 21(21): 1491-1494

Bhatia, A., 1997: Power, equity, gender, and conflicts in common property resources in the Hindu Kush-Himalayas. Presenting the historical perspective and political economy of common property resources' management and its associated issues within the Hindu Kush-Himalayas, the paper argues that the last ten years has seen increased commitment by the stat...

Natland J., 1977: Composition of basaltic rocks recovered at sites 367 and 368, Deep Sea Drilling Project, near the Cape Verde Islands. Initial Reports of the Deep Sea Drilling Project 41: 1107-1112

Heriberta Castaños; Cinna Lomnitz, 2003: Disasters at the interface of nature and society provoke thought. Eos, Transactions American Geophysical Union 84(47): 521

Saranummi, N., 2013: In the spotlight: Health information systems. IEEE Reviews in Biomedical Engineering 6: 21-23

Walker, L.O., 1989: Stress process among mothers of infants: preliminary model testing. Maternal employment, cesarean birth, and infant difficultness were used to test the mediating effect of perceived stress and the stress-buffering role of health practices on maternal identity. One hundred seventy-three mothers returned a parenting...