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Pharmacokinetics of triptolide. Development and application of a high performance liquid chromatographic method for quantitation of triptolide in plasma

, : Pharmacokinetics of triptolide. Development and application of a high performance liquid chromatographic method for quantitation of triptolide in plasma. Journal of Liquid Chromatography & Related Technologies 22(9): 1355-1366, May

In order to evaluate the bioavailability and study the pharmacokinetics of triptolide, an HPLC method was developed for the quantitative determination of this diterpenoid in plasma. The procedure for the plasma assay employed liquid - liquid extraction with chloroform followed by high speed centrifugation. The UV absorbance of the effluent was monitored at 218 nm. An internal standard (acetophenone) was used to calibrate injection and instrument reaction errors. The proposed methodology is sensitive, rapid, and reproducible. The limit of quantitation is 0.005 mg/L in plasma (0.05 mg/L in final solution) and a linear range of determination is observed over the concentration of 0.05 mg/L to 30 mg/L. The inter- and intraday coefficients of variation for the assay of triptolide in plasma were < 16.82 % at low concentration (0.005-0.076 mg/L) and < 8.05% at high concentration (0.152-5.000 mg/L). Recovery of triptolide in plasma is greater than 96.72%.


DOI: 10.1081/JLC-100101737

Other references

Thorgeirsson S.S., 1975: Induction by poly cyclic hydro carbons of aryl hydro carbon benzo a pyrene hydroxylase in regenerating liver of inbred strains of mice. Pharmacologist: 249

Lemke, A.A.; Smith, M.E.; Wolf, W.A.; Trinidad, S.Brown.; Boughman, J.; Burke, W.; Dressier, L.; Edwards, K.; Freeman, W.; Gerson, N.; Juengst, E.; Lewis, S.; Marshall, P.; O'Rourke, P.Pearl.; Ponsaran, R.; Press, N.; Griffin, M.Quinn.; Starks, H.; Wiesner, G., 2011: Broad data sharing in genetic research: views of institutional review board professionals. Irb 33(3): 1-5

Anon, 1974: Anatomy of the cervical spine. Bailey, Robert Wayneervical Spine. Viii 263p. Illus. Lea And Febiger: Philadelphia, Pa., U.s.a. 10-28

Anonymous, 2012: Dimerization and protease resistan New insight into the function of PR-. The group 1 pathogenesis-related (PR-1) proteins have long been considered hallmarks of hypersensitive response/defense pathways in plants, but their biochemical functions are still obscure despite resolution of the NMR/X-ray structures of several...

Ciocatto, E., 1960: Applications and results of the modern technics of anesthesiology and resuscitation in specialized otorhinolaryngological surgery. Minerva Otorinolaringologica 10: 1-5

Darcan, N.; Budak, D.; Kantar, M., 2005: Characterization of goat production in East Mediterranean Region of Turkey. This study reports on the present situation of goat farming in the mountainous are of Turkey. Data that was presented where gathered from 100 goat farmers in three geographical areas (North, Middle, and West of the Adana district). From the conduc...

Anonymous, 1988: To compare the cost per unit. Publication Virginia Cooperative Extension Service: 88 (348-557)

Staudt, K.A., 1987: Uncaptured or unmotivated? Women and the food crisis in Africa. Much theorizing has been done about the African food crisis. This paper reviews a gendered approach to agriculture, focusing on the implications of labour differentiation, incentives, and struggles over access to resources for agricultural develop...

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Biaglow, J.E.; Lavik, P.S.; Ferencz, N., 1969: Modification of radiation response through glucose-controlled respiration. Radiation Research 39(3): 623-633