geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Processing, disulfide pattern, and biological activity of a sugar beet defensin, AX2, expressed in Pichia pastoris


, : Processing, disulfide pattern, and biological activity of a sugar beet defensin, AX2, expressed in Pichia pastoris. Protein Expression and Purification 16(3): 377-387

AX2 is a 46-amino-acid cysteine-rich peptide isolated from sugar beet leaves infected with the fungus Cercospora beticola (Sacc.). AX2 strongly inhibits the growth of C. beticola and other filamentous fungi, but has little or no effect against bacteria. AX2 is produced in very low amounts in sugar beet leaves, and to study the protein in greater detail with respect to biological function and protein structural analysis, the methylotrophic yeast Pichia pastoris was used for large-scale production. The amino acid sequence, processing of the signal peptide, disulfide bridges, and biological activity of the recombinant protein were determined and compared with that of the authentic AX2. In P. pastoris, the protein was expressed with an additional N-terminal arginine. The disulfide bonding was found to be identical to that of the authentic AX2. However, when tested in in vitro bioassay, the biological activity of the recombinant protein was slightly lower than that measured for the authentic protein. Furthermore, the recombinant protein was significantly more sensitive to Ca(2+) than the authentic protein. This is most probably due to the extra arginine, since no other differences between the two proteins have been found.

US$19.90

PMID: 10425158

DOI: 10.1006/prep.1999.1085


Other references

Teti, D.M.; Hess, C.Reiner.; O'Connell, M., 2005: Parental perceptions of infant vulnerability in a preterm sample: prediction from maternal adaptation to parenthood during the neonatal period. The present longitudinal study of African-American mothers of preterm infants tested the hypothesis that the quality of maternal adaptation to parenthood before infant discharge from the hospital is predictive of maternal perceptions of infant vul...

Krekeler, G.; Lenz, P., 1975: The uses of self-cutting parapulpal metal pins. Zwr 84(18): 814-817

Anonymous, 1924: Report of the Department of Agriculture, Surinam, for the year 1923. The sections on the Agricultural Experiment Station, Diseases, and the phytopathological service (pp. 20-34), contributed by G. Stahel, contain the following references of interest. The obscure disease of the coffee shade tree Erythrina glauca des...

Pelisek J.; Sekaninova D., 1977: Partial results of research of soil moisture content in surface layer soils on the Doupe experimental area on the Czeckomoravska vrchoviny Highland. Zpravy Geografickeho Ustava CSAV 14(7-8): 203-206

Jing, L-Qing.; Yin, J.; Hou, G-Hui.; Liu, W.; Lin, Z-Yang.; Niu, H-Ben., 2014: High sensitivity conherent anti-stokes Raman scattering for linearly probing low concentration materials. The authors made a theoretical analysis and experiment research on the relation of time-resolved coherent anti-Stokes Raman scattering (T-CARS) intensity and the sample concentrations in this paper. It was proved experimentally that the T-CARS int...

Kapur, S., 1977: Diseases of citrus and their control. Progressive farming: 13 (5) 9-10

Sajo, K., 1898: Zur Lebensweise von Lyda erythrocephala L. und Lyda stellata Christ. Forstliche Zeitschrift Munchen, VII 237-247

Clark J.D.; Asfaw B.; Assefa G.; Harris J.W.K.; Kurashina H.; Walter R.C.; White T.D.; Williams M.A.J., 1984: Paleo anthropological discoveries in the middle awash valley ethiopia. A survey of Ethiopia's Middle Awash Valley in 1981 yielded new and significant archaeological, paleontological [associated mammalian fossils], geological and geochronological results. Included in the new discoveries are hominid fossils from t...

Shirley; Benedict; Louie, 1996: Excitons in solid C60. Physical Review. B, Condensed Matter 54(15): 10970-10977

Schiroli, G., 2005: Single-tooth implant restorations in the esthetic zone with PureForm ceramic crowns: 3 case reports. The anterior maxillary jaw is frequently called the "esthetic zone" because of its high visibility and influence on facial appearance. Implant placement and single-tooth restoration in this region can present some esthetic challenges for...