geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Production of hydroxyl radical by the synergistic action of fungal laccase and aryl alcohol oxidase


, : Production of hydroxyl radical by the synergistic action of fungal laccase and aryl alcohol oxidase. Archives of Biochemistry and Biophysics 383(1): 142-147

A mechanism for the production of hydroxyl radical (cntdotOH) during the oxidation of hydroquinones by laccase, the ligninolytic enzyme most widely distributed among white-rot fungi, has been demonstrated. Production of Fenton reagent (H2O2 and ferrous ion), leading to cntdotOH formation, was found in reaction mixtures containing Pleurotus eryngii laccase, lignin-derived hydroquinones, and chelated ferric ion. The semiquinones produced by laccase reduced both ferric to ferrous ion and oxygen to superoxide anion radical (O2.-). Dismutation of the latter provided the H2O2 for cntdotOH generation. Although O2.- could also contribute to ferric ion reduction, semiquinone radicals were the main agents accomplishing the reaction. Due to the low extent of semiquinone autoxidation, H2O2 was the limiting reagent in Fenton reaction. The addition of aryl alcohol oxidase and 4-methoxybenzyl alcohol (the natural H2O2-producing system of P. eryngii) to the laccase reaction greatly increased cntdotOH generation, demonstrating the synergistic action of both enzymes in the process.

US$19.90

PMID: 11097187

DOI: 10.1006/abbi.2000.2053


Other references

Maines-Bandiera, S.L.; Auersperg, N., 1997: Increased E-cadherin expression in ovarian surface epithelium: an early step in metaplasia and dysplasia?. Ovarian surface epithelium (OSE), the source of common epithelial carcinomas, is a simple mesothelium that during carcinogenesis acquires complex epithelial characteristics normally found in tubal, endometrial, and endocervical epithelia. These ch...

Globus, A.M.; Petrova, Z.M.; Ostapenko, N.S., 1992: The influence of water structure in marginal layers and of binary electric layer on the water-bentonite interaction. The effects of adding sucrose to water and CaCl2 solutions on the swelling pressure of bentonite were studied. Sucrose was shown to destroy the structure of water layers adjacent to the solid phase.

Cartin Leiva, N., 1987: Theoretical and methodological aspects for analysis of the reproductive strategy of peasants' families: the case of Orosi. The study proposes a critical revision of the concept of 'survival strategy' for peasant communities. (Other sources describe the above as 'the strategy of existence'). The peasant community of small coffee producers in Orosi d...

Jensen C.M.; Leklem J.E., 1978: Deoxy cytidine excretion in vitamin b 6 or pantothenic acid deficient rats. Federation Proceedings 37(3): 448

Lewis, P.D.; Fulop, Z.; Hajos, F.; Balazs, R.; Woodhams, P.L., 1977: Neuro glia in the internal granular layer of the developing rat cerebellar cortex. In the 1st wk after birth, a proliferating cell population identifiable as astrocytes was a prominent constituent of the developing internal granular layer of the rat cerebellar cortex. This population became less prominent in the 2nd postnatal wk...

Shetty, G.; Meistrich, M.L., 2000: Testosterone inhibits the differentiation of spermatogonia in juvenile spermatogonial depletion mice through an androgen receptor mediated process. Biology of Reproduction 62(Supplement 1): 108-109

Caan, W., 2002: Pool research budgets, don't split them up. NlmCategory="UNASSIGNED">Congratulations on your excellent article on the important issue of research funding, showing nursing research has fared badly (analysis January 3).

Perutz, M., 1994: Polar zippers: their role in human disease. Ascaris hemoglobin consists of 8 subunits, each of which contains a C-terminal peptide with the sequence Glu-Glu-Lys-His repeated 4 times. When plotted on a beta-strand, this sequence leads to alternate lysines and glutamates on one side of the st...

Knight, L.C.; Parkman, H.P.; Brown, K.L.; Miller, M.A.; Trate, D.M.; Urbain, J.L.; Maurer, A.H.; Fisher, R.S., 1996: Altered gastric emptying and antral motility in normal women vs men A study using dynamic antral scintigraphy. Journal of Nuclear Medicine 37(5 SUPPL ): 244P

Roemer, M.I., 1970: General physician services under eight national patterns. American Journal of Public Health and the Nation's Health 60(10): 1893-1899