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Quantification of tissue factor pathway inhibitor in human seminal plasma and in human follicular fluid


, : Quantification of tissue factor pathway inhibitor in human seminal plasma and in human follicular fluid. Thrombosis Research 109(5-6): 329-332, March 15

Introduction: Tissue factor pathway inhibitor (TFPI) is a multivalent Kunitz-type serine proteinase inhibitor, which plays a central role in the extrinsic pathway of blood coagulation. It inhibits activated factor X directly and factor VIIa/tissue factor via a quaternary complex. The composition of human semen is governed by the ejaculatory mixing of sperm-rich epididymal fluid, with secretion provided by the accessory sex glands. It is composed of more than 30 proteins including coagulation and liquefaction proteins. Ovarian follicular fluid plays an important biological role in folliculogenesis and oocyte maturation, and it remains in a hypocoagulable state until ovulation. Materials and methods: TFPI levels were measured in ovarian follicular fluid gained from the punctured follicles of superovulated women (n=70), and, for the first time, in seminal plasma of 28 healthy ejaculate donors and 23 infertile patients with oligozoospermia, asthenozoospermia, or teratozoospermia. Results: TFPI concentrations determined in liquor folliculi (median 298 ng/ml, 90% range 109-648 ng/ml) were four times higher than the levels found in human blood of healthy individuals. TFPI concentrations in seminal plasma samples of infertile men were significantly reduced (median 2.20 ng/ml, 90% range 0.28-6.02 ng/ml, p<0.07) in comparison to healthy donors (median 3.55 ng/ml, 90% range 0.93-7.90 ng/ml). Conclusions: The high TFPI levels measured in the ovarian follicular fluid underline the physiological importance of this inhibitor for maintaining the hypocoagulable state. The decreased TFPI concentrations in seminal plasma of infertile men support the possible correlation between the coagulation properties of ejaculated semen and male fertility.

US$19.90

PMID: 12818258

DOI: 10.1016/s0049-3848(03)00210-x


Other references

Birettoni, F.; Porciello, F.; Caivano, D.; Arcelli, R.; Sforna, M.; Antognoni, M., 2008: Primary neuroendocrine carcinoma of the gallbladder in a dog. Veterinary Research Communications 32 Suppl 1: S239-S242

Rendall, R.; P.U.P.017950; A.C.mpass Plants B V, 2007: Geranium plant named 'Tanya Rendall'. A new and distinct cultivar of Geranium plant named 'Tanya Rendall', characterized by its outwardly spreading, trailing and mounded plant habit; brown-colored foliage; freely and uniformly flowering habit; long flowering period; purple v...

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Amorosi A.; Centineo M.C.; Colalongo M.L.; Pasini G.; Sarti G.; Vaiani S.C., 2003: Facies architecture and latest Pleistocene-Holocene depositional history of the Po Delta (Comacchio area), Italy. Integrated sedimentological and micropaleontological study of 16 cores and 137 piezocone penetration tests, approximately 40 m deep, in the Comacchio area enables the documentation of the depositional history of southeastern Po Plain in the last 3...

Paulus W.; Bancher C.; Jellinger K., 1991: Reproducibility of the neuropathological diagnosis of alzheimers disease. Neuropathology & Applied Neurobiology 17(6): 527

Ojetti, V.; Gasbarrini, A.; Pitocco, D.; Franceschi, F.; Torre, E.S.; Candelli, M.; Armuzzi, A.; Gabrielli, M.; Cremonini, F.; Danese, S.; Carloni, E.; Canducci, F., 1999: Efficacy of different eradication regimens in insulin dependent diabetes mellitus patients affected by Helicobacter pylori. Gastroenterology 116(4 PART 2): A270-A271, April

Dorlöchter, L.; Helgheim, V.; Røksund, O.D.; Rosendahl, K.; Fluge, G., 2004: Shwachman-Kulczycki score and resting energy expenditure in cystic fibrosis. Disease severity assessed by clinical scores in cystic fibrosis (CF) has been a topic of investigation for many years, although a correlation of clinical scores with resting energy expenditure (REE) has not been described yet. We aimed to assess d...

Takahashi, K., 1996: Relationships between the blacking phenomenon and norlignans of sugi (Cryptomeria japonica D. Don) heartwood I. A case of partially black heartwood. The relationship between the blackening phenomenon of sugi (Cryptomeria japonica) heartwood and norlignan content was investigated using a partially black heartwood. The moisture content, methanol extract amount, and pH value of the black heartwoo...

Molinuevo, H.; Vissac, B., 1972: Genetic variability of pre-washing growth in Charolais and Limousin cattle. Annales de genetique et de selection animale: (3) 423-444

Klima, F.; Hofmann, R., 1997: Proceedings of the 1st International Symposium on Physiology and Ethology of Wild and Zoo Animals. Berlin, Germany September 18-21, 1996. Zeitschrift fuer Saeugetierkunde 62: 1-248 (Supplementum 2)