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Quantitative histology by Laser Scanning Cytometry

, : Quantitative histology by Laser Scanning Cytometry. Journal of Histotechnology 24(1): 59-62, March

The Laser Scanning Cytometer (LSC) is a relatively new class of instrument that combines features of both flow and image cytometry. The LSC is capable of measuring multicolor fluorescence, light scatter, and the location of cells fixed to a glass slide. It is capable of automatically measuring a large number of cells with data displayed in real time. In addition, cell location is recorded so that cells of interest can be relocated for morphological examination and classification. The LSC is capable of measuring a wide variety of sample types, including thin preps, cytospins, touch imprints, and tissue sections. In this brief review paper, the mechanics of laser scanning cytometry are examined along with potential applications to conventional histopathology.


DOI: 10.1179/his.2001.24.1.59

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