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Rapid selection of anti-hapten antibodies isolated from synthetic and semi-synthetic antibody phage display libraries expressed in Escherichia coli


, : Rapid selection of anti-hapten antibodies isolated from synthetic and semi-synthetic antibody phage display libraries expressed in Escherichia coli. FEMS Microbiology Letters 210(2): 257-261, 7 May

Antibody phage display libraries (Griffin and Tomlinson I) displaying antibody genes and maintained and amplified in Escherichia coli were used to isolate antibodies to the hapten target microcystin LR (1000 Da) conjugated to either bovine serum albumin or keyhole limpet haemocyanin. In competition enzyme-linked immunosorbent assay, bacterially expressed antibodies selected via the Griffin library showed at least 300 times greater sensitivity than those isolated from the Tomlinson library, for free microcystin. Bacterially expressed phage antibody libraries provide a rapid and relatively easy route for the selection of monoclonal antibodies specific for even the most difficult of antigenic targets such as free haptens.

US$19.90

PMID: 12044684

DOI: 10.1111/j.1574-6968.2002.tb11190.x


Other references

White, J.G.; Burris, S.M.; Hasegawa, D.; Johnson, M., 1984: Micropipette aspiration of human blood platelets: a defect in Bernard-Soulier's syndrome. Previous reports have suggested that platelets from patients with Bernard-Soulier's syndrome (BSS) are not giant cells. Rather, they are normal-sized in suspension, but spread out on glass slides more readily than control cells, yielding the...

Gillum R.F.; Feinleib M.; Margolis J.R.; Fabsitz R., 1975: Prodromata of sudden cardiac death relatives of patients as sources of data. American Journal of Epidemiology 102(5): 448-449

Andrade Tarozzo, L.Silva.; Chiarello D.M.ttos, M.da.Gloria.; Faria Ribeiro, R.; Semprini, M., 2003: Comparison of retentive systems for composites used as alternatives to porcelain in fixed partial dentures. The bond strength between framework metals and composites, used as alternatives to porcelain, requires further study because adhesive failures can promote microleakage, discoloration, and stains in composites. The aim of this in vitro study was to...

Ferreira, S.A.; Webster, R.K., 1974: Evaluation and inheritance of virulence in Sclerotium oryzae. When a population of 69 single conidial isolates of Leptosphaeria salvinii from California was evaluated in tests on rice seedlings a large range of variation in virulence was indicated. When single-ascospore progeny of crosses between isolates di...

Han, S.; Jerome, J.A.; Gregory, A.D.; Mallampalli, R.K., 2017: Cigarette smoke destabilizes NLRP3 protein by promoting its ubiquitination. Cigarette smoke suppresses innate immunity, making smokers more susceptible to infection. The NLRP3 inflammasome is a multi-protein complex that releases interleukin (IL) -1β and IL -18. These cytokines are critical for a timely host response to...

Luck, J.M., 1938: The San Diego Meeting Of The Pacific Division. Science 88(2279): 195-204

Cerklewski, F.L., 1984: Postabsorptive effect of increased dietary zinc on toxicity and removal of tissue lead in rats. Weanling male albino rats were fed a diet containing 12 ppm zinc and 200 ppm lead for 3 weeks. At the end of this time a representative number of samples were collected to determine tissue zinc and lead, inhibition of the lead-sensitive liver enzy...

Ladabaum, U.; Song, K.; Fendrick, A.Mark., 2004: Colorectal neoplasia screening with virtual colonoscopy: when, at what cost, and with what national impact?. When optimized, virtual colonoscopy may be highly sensitive for colorectal neoplasia. We evaluated the effectiveness and cost-effectiveness of virtual colonoscopy screening (VC) vs. colonoscopy screening (COLO) and the potential impact at the nati...

Anonymous, 1935: David Roxburgh, M.B., C.M. British Medical Journal 2(3912): 1286-1286

Focas J.H., 1967: The contribution of observations carried out from the earth in the direct exploration of the planets. Pages 7-22 1967