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Reliable detection of respiratory syncytial virus infection in children for adequate hospital infection control management


, : Reliable detection of respiratory syncytial virus infection in children for adequate hospital infection control management. Journal of Clinical Microbiology 39(9): 3135-3139

By using a rapid test for respiratory syncytial virus (RSV) detection (Abbott TestPack RSV), a number of patients were observed, showing repeatedly positive results over a period of up to 10 weeks. A prospective study was initiated to compare the rapid test with an antigen capture enzyme immunoassay (EIA) and a nested reverse transcriptase PCR (RT-PCR) protocol for detection of RSV serotypes A and B. Only respiratory samples from children exhibiting the prolonged presence of RSV (> or =5 days) as determined by the rapid test were considered. A total of 134 specimens from 24 children was investigated by antigen capture EIA and nested RT-PCR. Using RT-PCR as the reference method, we determined the RSV rapid test to have a specificity of 63% and a sensitivity of 66% and the antigen capture EIA to have a specificity of 96% and a sensitivity of 69% for acute-phase samples and the homologous virus serotype A. In 7 (29%) of 24 patients, the positive results of the RSV rapid test could not be confirmed by either nested RT-PCR or antigen capture EIA. In these seven patients a variety of other respiratory viruses were detected. For general screening the RSV rapid test was found to be a reasonable tool to get quick results. However, its lack of specificity in some patients requires confirmation by additional tests to rule out false-positive results and/or detection of other respiratory viruses.

US$19.90

PMID: 11526141

DOI: 10.1128/JCM.39.9.3135-3139.2001


Other references

Vugrek, O.; Beluzić, R.; Nakić, N.; Mudd, S.Harvey., 2009: S-adenosylhomocysteine hydrolase (AHCY) deficiency: two novel mutations with lethal outcome. This paper reports studies of two novel, allelic missense mutations found in the S-adenosylhomocysteine hydrolase (AHCY) gene from a new case of AHCY deficiency in an infant girl who died at age four months. The mutations lead to replacement of ar...

Makhimov F.Y.; Savel yeva L.A., 2000: Humid Holocene stages in northwestern Russia. Three sites from Leningrad Province, Novgorod region, and Karelian Isthmus were studied by the palynological method, and radiocarbon data in each 10-cm layer of the sections were obtained. It was presumed to use an original index of humidification...

Lindop P.H., 1986: The deduction of approximate values of sediment depth from propagation loss measurements. Pages 243-252 1986

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Kaiser, L.; Grönlund, H.; Sandalova, T.; Ljunggren, H-Gustaf.; Schneider, G.; van Hage-Hamsten, M.; Achour, A., 2003: Production, crystallization and preliminary crystallographic study of the major cat allergen Fel d 1. The domestic cat (Felis domesticus) is an important cause of allergic disease worldwide. The major cat allergen 1 (Fel d 1) has been expressed in Escherichia coli, purified and refolded in a soluble form. Crystals of Fel d 1 were obtained in 13% 2...

Gottikh B.P., 1976: The study of the stimulating mechanism of the peptidyl donor activity of 2 3 o n formylmethionyl amp in peptidyl transferase of escherichia coli ribosome. S Letters: 101-104

Benoit, D.; Simard, Y.; Fortier, L., 2008: Hydroacoustic detection of large winter aggregations of Arctic cod Boreogadus saida at depth in ice-covered Franklin Bay Beaufort Sea. Journal of Geophysical Research 113.C6

Laniez, C.; Corsin, L., 1961: The association of anethocurarium and hydroxydione in the treatment of tetanus. Apropos of a case. Anesthesie, Analgesie, Reanimation 18: 349-355

Blada, I., 1997: Stone pine (Pinus cembra L.) provenance experiment in Romania. I. Nursery stage at age 6. A stone pine (P. cembra) provenance study was started in 1990 at a nursery near Sinaia, Romania, involving 7 seedlots from the Romanian Carpathians and 5 seedlots from the Austrian, Swiss and French Alps. After 6 years, total height growth, height...