geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Response to treatment with interferon-alfa in patients with chronic hepatitis C and high titers of -M2, -M4 and -M8 antimitochondrial antibodies


, : Response to treatment with interferon-alfa in patients with chronic hepatitis C and high titers of -M2, -M4 and -M8 antimitochondrial antibodies. Revista Espanola de Enfermedades Digestivas 91(3): 168-181

The prevalence of antimitochondrial antibodies (AMA) in chronic hepatitis C is 2%; titers of AMA are usually low (< 1:40). The prevalence decreases to 0.5% when the results are verified by determination of the M2 subtype (anti-M2, ELISA). In patients in whom both hepatitis C virus (HCV) and AMA are present, the therapeutic decision to give interferon-alfa is complicated, because AMA may be "real", and if it reflects primary biliary cirrhosis, cholestasis can be triggered or exacerbated. This does not occur when AMA positivity results from induction by hepatotropic C virus; however, this is rarely the case when AMA titers are high (> 1:160). OBJECTIVE: to undertake a preliminary analysis of the submitochondrial profile of AMA in three patients with chronic hepatitis C and positive AMA titers (> 1:160). METHODS: we determined antibodies to submitochondrial particles (subtypes) -M2, -M4 and -M8 by ELISA, complement binding (CB) and western immunoblotting with Immunoblot-M2 or WIB-M2 (immunoreactive bands). RESULTS: two patients were positive for mitochondrial subtypes by ELISA (IgG/IgM subclass) and CB (ELISA M2 470/365 in patient 1 and 600/1370 in patient 2; M4 490/1200 in patient 2. CB M2 1:128, M4 1:64, M8 1:64 in patient 1, M2 1:128 in patient 2). Immunoreactive epitopes (bands) were detected with WIB-M2 for 70, 56, 51, 45 and 36-kDa molecules. Interferon-alfa treatment was unsuccessful, with biochemical exacerbation of cholestasis. In contrast, the patient with no submitochondrial particles according to ELISA, CB and WIB-M2 results responded favorable to this drug. CONCLUSION: these preliminary results suggest that analyses to detect antibodies to submitochondrial particles (-M2, -M4 and -M8 subtypes) and -M2-immunoreactive epitopes in patients with chronic hepatitis C and AMA titers > 1:160 facilitates the diagnosis of primary biliary cirrhosis, and establishes a contraindication for treatment with interferon-alfa despite the presence of HCV infection.

US$29.90

PMID: 10231308


Other references

Shida, Y.; Hakariya, T.; Miyata, Y.; Sakai, H., 2017: Three cases of nonmetastatic prostate cancer treated successfully with primary intermittent androgen deprivation therapy over 10 years. We report three cases of nonmetastatic prostate cancer treated effectively with long-term primary intermittent androgen deprivation (IAD). IAD is not a standard therapy for patients with nonmetastatic prostate cancer. However, based on our experie...

Gujer, W., 2006: Activated sludge modelling: past, present and future. Model complexity in activated sludge modelling has increased over 30 years in parallel with the computational power of computers. Today, the complexity of biokinetics has reached a practical limit. Future advances may be in the direction of enhanc...

Garofalakis, A.; Zacharakis, G.; Meyer, H.; Economou, E.N.; Mamalaki, C.; Papamatheakis, J.; Kioussis, D.; Ntziachristos, V.; Ripoll, J., 2007: Three-dimensional in vivo imaging of green fluorescent protein-expressing T cells in mice with noncontact fluorescence molecular tomography. Given that optical tomography is capable of quantitatively imaging the distribution of several important chromophores and fluorophores in vivo, there has been a great deal of interest in developing optical imaging systems with increased numbers of...

Kalady, M.F.; Onaitis, M.W.; Emani, S.; Abdel Wahab, Z.; Tyler, D.S.; Pruitt, S.K., 2002: Sequential maturation stimuli enhance antigen-specific immunogenicity of RNA-transfected dendritic cells. Proceedings of the American Association for Cancer Research Annual Meeting 43: 677, March

Lindgren, V., 1978: Mapping of a genetic locus that affects glycerol 3-phosphate transport in Bacillus subtilis. Two types of fosfomycin-resistant mutants of Bacillus subtilis were isolated. Mutants of the first type (GlpT mutants) were resistant to at least 200 microgram of fosfomycin per ml and failed to take up exogenous glycerol 3-phosphate. Mutants of t...

Schleiffer, R.; Raul, F., 1996: Prophylactic administration of L-arginine improves the intestinal barrier function after mesenteric ischaemia. Background: Ischaemia/reperfusion (LR) of the intestine causes mucosal injury associated with a high death rate in rats. Aim: To investigate whether nitric oxide (NO) might be implicated in the recovery of the intestinal mucosa after ischaemic ins...

Schmidt-Nielsen, B.; Schmidt-Nielsen, K., 1949: The water economy of desert mammals. Desert mammals are divided into two classes, those which store water and need periodic replenishment, and those which can exist on the meagre supply available. The desert rats under observation were of the latter class, living on diets of dry seed...

Anonymous, 2010: The 10 security domains (updated). Journal of Ahima 81(2): 57-61

Cristino, L.; Pica, A.; Della Corte, F.; Bentivoglio, M., 2000: Plastic changes and nitric oxide synthase induction in neurons that innervate the regenerated tail of the lizard Gekko gecko: 1. Response of spinal motoneurons to tail amputation and regeneration. The lizard tail regenerates after autotomy or amputation. After horseradish peroxidase injections in the regenerate, motoneurons were retrogradely labeled only in the three spinal segments rostral to the amputation, whose spinal nerves are severed...

Tremblay, Aé.J.; Lamarche, Bît.; Ruel, I.L.; Hogue, J-Charles.; Deshaies, Y.; Gagné, C.; Couture, P., 2005: Effects of fenofibrate on apolipoprotein kinetics in patients with coexisting dysbetalipoproteinemia and heterozygous familial hypercholesterolemia. Dysbetalipoproteinemia (dysb) and familial hypercholesterolemia (FH) are two genetic disorders giving rise to severe disturbances of lipid homeostasis and premature atherosclerosis. The co-occurrence of both metabolic abnormalities is very rare an...