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Rhinovirus replication causes RANTES production in primary bronchial epithelial cells

, : Rhinovirus replication causes RANTES production in primary bronchial epithelial cells. American Journal of Respiratory Cell and Molecular Biology 20(6): 1220-1228

The mechanisms by which rhinovirus (RV) infections produce lower airway symptoms in asthmatic individuals are not fully established. To determine effects of RV infection on lung epithelial cells, primary human bronchial epithelial (BE) cells were infected with either RV16 or RV49, and viral replication, cell viability, and cell activation were measured. Both viral serotypes replicated in BE cells at 33 degrees C (DeltaTCID50 / ml = 2 to 2.5 log units) and at 37 degrees C (DeltaTCID50 /ml = 1.6 log units), but only high doses of RV49 (10(6) TCID50 /ml) caused cytopathic effects and reduced cell viability. In addition, regulated on activation, normal T cells expressed and secreted (RANTES) secretion was increased in epithelial cells infected with RV16 or RV49 (243 and 398 pg/ml versus 13 pg/ml uninfected control cells), and a similar pattern was seen for RANTES messenger RNA. RV infection also caused increased secretion of interleukin-8 and granulocyte macrophage colony-stimulating factor, but did not alter expression of either intercellular adhesion molecule-1 or human leukocyte-associated antigen-DR. These observations suggest that RVs can replicate in lower airway cells in vivo, and support epidemiologic studies that link RV with lower respiratory illnesses. Further, RV-induced secretion of RANTES and other cytokines could trigger antiviral immune responses in vivo, but these effects could also contribute to the pathogenesis of respiratory symptoms in subjects with asthma.


PMID: 10340941

DOI: 10.1165/ajrcmb.20.6.3261

Other references

Alghisi, P.; D.L.nna, P.; Magro, P., 1971: Comparison and characterization of the polyphenoloxidase activity in healthy Maize plants, susceptible and resistant to Ustilago zeae. The total polyphenoloxidase capacity and the kinetic constants Km and Vmax on several phenolic substrates were determined to compare the polyphenoloxidase activity of vars. susceptible (Marano vicentino) and resistant (Funk's G-77) to U. zeae...

Schary, E.G., 1943: Hand raising of budgies. Individual care is necessary for proper training of pets and encouraging talking. Pine nuts-are a favored training tid-bit.

Kumar, R.M.rck, B., 1993: On common ground (book review). Journal of Forestry 91

Millan, Jesus., 1994: Hallazgo de un nido de puesta comunal de Malpolon monspessulanus con datos de dos puestas. Boletin de la Asociacion Herpetologica Espanola, 5: 29-31

Rubin, G.; Haddad, E.; Ben-Haim, T.; Elmalach, I.; Rozen, N., 2011: Bilateral, simultaneous rupture of the quadriceps tendon associated with simvastatin. Israel Medical Association Journal 13(3): 185-186

Lucas C.L.; H.B.; Henry G.W.; Ferreiro J.I.; Frantz E.G.; Wilcox B.R., 1990: Characterization of pulmonary artery blood velocity patterns in lambs. Biorheology 27(1): 82

Smirnov, A.D., 1975: Correspondence of diastolic fall of pressure in the pulmonary artery to exponent and its use for calculation of hemodynamic resistance of the lesser. In accordance with the principles of theoretical hemodynamics it is established that the diastolic fall of the pulmonary artery pressure corresponds to the exponent both in normals, and in patients with the main types of pathology. Having determin...

Clifford, C.; Walker, J.1; Keirans, J., 1983: Clarification of the status of Rhipicephalus kochi Donitz, 1905 (Ixodoidea, Ixodidae) Taxonomy, animal hosts, South Africa. Onderstepoort journal of veterinary research 50(1): 77-89

Cayouette, R., 1985: Les gros-becs errants sont-ils fideles a votre mangeoire?. Carnets de Zoologie, 451: 15

Dayou, F.; Tchang-Brillet, W-U.L.; Monnerville, M., 2005: Quasi-classical trajectory study of Si+O2-->SiO+O reaction. Quasi-classical trajectory calculations for the Si(3P)+O2(X 3Sigmag-)-->SiO(X 1Sigma+)+O(1D) reaction have been carried out using the analytical ground 1A' potential energy surface (PES) recently reported by Dayou and Spielfiedel [J. Chem....