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Saturated non-esterified fatty acids stimulate de novo diacylglycerol synthesis and protein kinase c activity in cultured aortic smooth muscle cells

, : Saturated non-esterified fatty acids stimulate de novo diacylglycerol synthesis and protein kinase c activity in cultured aortic smooth muscle cells. Diabetologia 44(5): 614-620

Aims/hypothesis: Insulin resistance is linked with a cluster of multiple risk factors and excessive acceleration of atherosclerosis. The underlying mechanism is not, however, fully understood. Methods: To determine the link between insulin resistance and altered vascular function, we focused on the effect of various non-esterified fatty acids on diacylglycerol-protein kinase C pathway and mitogen-activated protein kinase activity in cultured aortic smooth muscle cells. Results: Incubation of the cells with saturated non-esterified fatty acids (200 mumol/l) for 24 h, such as palmitate or stearate, induced a significant increase in diacylglycerol concentrations by about fivefold or eightfold, respectively, whereas oleate induced a slight increase in diacylglycerol concentrations by 1.8-fold and arachidonate induced none. In addition, the increased diacylglycerol concentrations induced by palmitate were completely restored to control concentrations by triacsin C, acyl-CoA synthetase inhibitor. These results suggest that saturated non-esterified fatty acids may increase diacylglycerol concentrations through de novo pathway by stepwise acylation. In parallel with the increased diacylglycerol, incubation of the cells with saturated non-esterified fatty acids significantly induced the activation of protein kinase C and mitogen-activated protein kinase. The palmitate-induced increase in mitogen-activated protein kinase activity was restored to control concentrations by GF109203X (5 cntdot 10-7 mol/l), a specific protein kinase C inhibitor, suggesting a protein kinase C-dependent activation of mitogen-activated protein kinase. Conclusion/interpretation: Saturated non-esterified fatty acids induced an increase in de novo diacylglycerol synthesis and subsequent activation of protein kinase C and mitogen-activated protein kinase in cultured aortic smooth muscle cells. This could contribute to the altered vascular functions in the insulin resistant state.


PMID: 11380080

DOI: 10.1007/s001250051668

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Chen, C-Chen.; Jeon, S-Min.; Bhaskar, P.T.; Nogueira, V.; Sundararajan, D.; Tonic, I.; Park, Y.; Hay, N., 2010: FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor. FoxO transcription factors and TORC1 are conserved downstream effectors of Akt. Here, we unraveled regulatory circuits underlying the interplay between Akt, FoxO, and mTOR. Activated FoxO1 inhibits mTORC1 by TSC2-dependent and TSC2-independent mec...

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Erwin, J.; Mitchell, G., 1973: Analysis of Rhesus monkey vocalizations: maturation-related sex differences in clear-cell frequency. American Journal of Physical Anthropology 38(2): 463-467

Landaverde, C.; Saab, S., 2008: Deciding liver transplant candidacy: tools of the trade. Liver International 28(9): 1186-1189

Wdzieczak-Bakala, J.; Grillon, C.; Robinson, S.; Riches, A.; Carde, P.; Lenfant, M., 1993: Catabolism of the tetrapeptide N-Ac-Ser-Asp-Lys-Pro (AcSDKP), an inhibitor of hematopoietic stem cell (CFU-S) proliferation, following in vitro incubation with hematopoietic tissues from normal and leukemic mice. The comparative degradation of N-Ac-Ser-Asp-Lys-Pro (AcSDKP), a negative regulator controlling the proliferation of the hematopoietic pluripotent stem cell, was investigated following incubation with plasma, bone marrow and spleen cells from norma...

Aubert E.J., 1978: Relevance of international field year of the great lakes. Sladecek, V (Ed ) Internationale Vereinigung Fuer Theoretische Und Angewandte Limnologie Verhandlungen International Association Of Theoretical And Applied Limnology, Proceedings, Vol 20 Part 1 And 2 Copenhagen, Denmark, Aug 8, 1977 Iv+748p ; Viii+708p Illus Maps E Schweizerbart'sche Verlagsbuchhandlung: Stuttgart, West Germany Isbn 3-510-54013-1 (Part 1); Isbn 3-510-54014-X (Part 2) 317-323

Jakicic, J.M.; Wing, R.R.; Winters, C., 2000: Exercise and long-term weight loss Is there an association with other weight loss behaviors?. Medicine & Science in Sports & Exercise 32(5 Suppl ): S300, May

Dickinson, J.E.; Godfrey, M.; Evans, S.F.; Newnham, J.P., 1997: Factors influencing the selection of analgesia in spontaneously labouring nulliparous women at term. We investigated the maternal characteristics, labour performance and delivery mode of 497 nulliparas entering labour spontaneously at term to identify the obstetric factors which influenced their choice of analgesia; 51.7% of these women used epid...

Hawke, J.C., 1963: The distribution of fatty acids between the alpha '- and beta -positions of the glycero-phospholipids of buttermilk. The phospholipids of buttermilk were fractionated on silicic acid columns to give cephalin, a mixture of amino-N phospholipids containing about 29% saturated fatty acids, and phosphatidyl choline which contained about 57% saturated fatty acids. Ce...