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Screening for abnormalities of the protein C anticoagulant pathway using the ProC Global assay. Results of a European multicenter evaluation


, : Screening for abnormalities of the protein C anticoagulant pathway using the ProC Global assay. Results of a European multicenter evaluation. Blood Coagulation & Fibrinolysis 11(5): 447-454

ProC Global is a new global clotting assay designed to evaluate the functionality of the protein C anticoagulant pathway. It is based on the ability of endogenous activated protein C, generated by activation of protein C by Protac(R), to prolong an activated partial thromboplastin time, and the results are expressed in protein C activation time normalized ratio (PCAT-NR), after normalization. This multicenter trial involving five European laboratories was designed in order to determine the ability of the ProC Global assay to distinguish patients with and without abnormalities of the protein C pathway. The PCAT-NR was significantly lower in the patients with a thrombotic history not on oral anticoagulant treatment (n = 627) than in the healthy controls (n = 148), even after exclusion from both groups of the patients with abnormality of the protein C pathway. Using receiver operator characteristics analysis, the cut-off level of PCAT-NR = 0.80 was found to provide the best sensitivity-specificity ratio. All the carriers of the factor V Leiden mutation (n = 73), as well as all the patients with activated protein C resistance (n = 42), had a PCAT-NR below 0.80. The ProC Global assay performed well in patients with combined defects (97.0%, n = 33) or protein C deficiency (91.3%, n = 46), but it failed to detect all of them, and one patient with combined defects as well as four patients with a low protein C level had a PCAT-NR above the cut-off level. The sensitivity of the assay for protein S deficiency (n = 58) was weak (only 69.0%) and, surprisingly, more than 40% of the 375 patients without any of these abnormalities of the protein C pathway had a PCAT-NR below the cut-off level.

US$19.90

PMID: 10937806

DOI: 10.1097/00001721-200007000-00008


Other references

Azuma, M., 1981: On the origin of Koayu, a landlocked form of amphidromous Ayu-fish, Plecoglossus altivelis. Proceedings International Association of Theoretical and Applied Limnologyub 1981) 21(2): 1291-1296

Gitlow, S.E.; Mendlowitz, M.; Wilk, E.K.; Wilk, S.; Wolf, R.L.; Bertani, L.M., 1968: Excretion of catecholamine catabolites by normal children. Journal of Laboratory and Clinical Medicine 72(4): 612-620

Rodriguez-Arnao, M.D.; Gomez-Pan, A.; Rainbow, S.J.; Woodhead, S.; Comaru-Schally, A.M.; Schally, A.V.; Meyers, C.A.; Coy, D.H.; Hall, R., 1981: Effects of prosomatostatin on growth hormone and prolactin response to arginine in man. Comparison with somatostatin. The effects of the hypothalamic 28 aminoacid peptide prosomatostatin (Pro-SS) on arginine-induced growth hormone (GH) and prolactin (PRL) release and blood glucose levels in man are compared with those obtained after an equimolar dose of somatosta...

Estebe, J.P.; Ecoffey, C.; Dollo, G.; L.C.rre, P.; Chevanne, F.; L.V.rge, R., 2002: Bupivacaine pharmacokinetics and motor blockade following epidural administration of the bupivacaine-sulphobutylether 7-beta-cyclodextrin complex in sheep. European Journal of Anaesthesiology 19(4): 308-310

Park C H.; Okos M.R.; Wankat P.C., 1989: Simultaneous product separation by fermentation gas during abe fermentation. Abstracts of Papers American Chemical Society 198: MBTD 37

Kramkowska, M.; Grzelak, T.; Czyżewska, K., 2014: Benefits and risks associated with genetically modified food products. Scientists employing methods of genetic engineering have developed a new group of living organisms, termed 'modified organisms', which found application in, among others, medicine, the pharmaceutical industry and food distribution. The i...

Lager, I.; Looger, L.L.; Hilpert, M.; Lalonde, S.; Frommer, W.B., 2006: Conversion of a putative Agrobacterium sugar-binding protein into a FRET sensor with high selectivity for sucrose. Glucose is the main sugar transport form in animals, whereas plants use sucrose to supply non-photosynthetic organs with carbon skeletons and energy. Many aspects of sucrose transport, metabolism, and signaling are not well understood, including t...

Benada, J., 1969: Brown patches on leaves of Barley in the relationship to powdery mildew. Initiation of powdery mildew growth from brown patches and plasmolysis experiments support the view that these patches contain living cells and therefore cannot be considered as necrotic host reactions .

Elliott, A.M.; McAteer, A.; Hannaford, P.C., 2012: Incongruous consultation behaviour: results from a UK-wide population survey. Symptom characteristics are strong drivers of care seeking. Despite this, incongruous consultation behaviour occurs and has implications for both individuals and health-care services. The aim of this study was to determine how frequently incongruo...

Ball, M.J., 1993: A New IMIA Tradition. Yearbook of Medical Informatics: 1-2