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Sensitivity to vincristine-induced apoptosis in K562 and K562/VCR


, : Sensitivity to vincristine-induced apoptosis in K562 and K562/VCR. Journal of Shanghai Medical University 26(3): 191-193, May

Purpose To study the sensitivity of human leukemic cell line K562 and vincristine-resistant cell variant K562/VCR to vincristin-induced apoptosis and explore the effect of antiapoptosis on the multidrug resistance of leukemia. Methods K562 and K562/VCR cells were treated with vincristine for 24 hours at the concentration of 0,0.001,0.010 mug/ml respectively. Apoptosis was detected with flow cytometry(FCM), TdT-mediated dUTP nick end labeling (TUNEL), and ELISA. Results After the treatment without vincristine, the apoptotic rate of K562 and K562/VCR was (4.10 +- 0.17) % and (3.61 +- 0.69) % respectively and at the concentration of 0.001 mug/ml, the rate was (13.30 +- 3.74) % and (9.06 +- 4.24) % respectively, as determined by FCM. Statistical analysis showed that there was no significant difference in apoptotic rate between K562 and K562/VCR at the above concentrations(n = 3, P > 0.05). But as the concentration of vincristine was increased to 0.010 mug/ml, the apoptotic rate of K562(36.48 +- 6.70) % was significantly higher than that of K562/VCR(10.45 +- 3.71) % (n = 3, P < 0.01). Similar results were also obtained with TUNEL and ELISA. Conclusions K562 and K562/VCR cells have different sensitivity to vincristine-induced apoptosis. The resistance of drug-resistant leukemic cells to drug-induced apoptosis may play an important role in multidrug resistance of leukemia.

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Other references

Imamura, Y.; Harada, H.; Saiki, H., 1974: Further study on the development of the bordered pit in coniferous tracheids. Electron microscopy of direct carbon replicas of the bordered-pit membrane of differentiating early-wood tracheids from Pinus densiflora, Cryptomeria japonica and Chamaecyparis obtusa confirmed the sequences of changes recorded previously for P. d...

Poli, F.; Pancaldi, S.; Dall'Olio, G.; Fasulo, M.P., 1995: Morphogenetic effects induced by spermine and ruthenium red in yeasts. Spermine (Sp) produces growth inhibition and wall malformation in Saccharomyces cerevisiae in response to oversynthesis of beta-glucans and chitin. The effect is related to the polycation nature of the molecule. In the present work, to verify this...

Ikeda, K.; Kato, T.; Suzuki, T.; Achiwa, K., 1998: Design and synthesis of haptens for application to the preparation of chiral 1,4-dihydropyridines. Lipase-catalyzed enzymatic hydrolysis of dimethyl esters of 1,4-dihydropyridines to the monoester, which is an important intermediate for the synthesis of optically active 1,4-dihydropyridines, does not proceed directly. This paper describes the d...

Hamauzu, Y.; Chachin, K.; Ding, C.K.i; Kurooka, H., 1997: Differences in surface color, flesh firmness, physiological activity, and some components of loquat fruits picked at various stages of maturity. To study the compositional and physiological difference of 'Mogi' loquat fruits, the fruits harvested at different maturity stages were analyzed for surface color, flesh firmness, sugar content, acid content, phenolic compounds content,...

Saeed, I.T.; Fletcher, C.D., 1990: Ectopic hamartomatous thymoma containing myoid cells. Histopathology 17(6): 572-574

Leger, M., 1921: Microfilaire sanguine du boeuf a la Guyane franYaise. Compte Rendu des Seances de la Societe de Biologie Paris, 84: 419 & 420

Leung, J.M.; Mayo, J.; Tan, W.; Tammemagi, C.Martin.; Liu, G.; Peacock, S.; Shepherd, F.A.; Goffin, J.; Goss, G.; Nicholas, G.; Tremblay, A.; Johnston, M.; Martel, S.; Laberge, F.; Bhatia, R.; Roberts, H.; Burrowes, P.; Manos, D.; Stewart, L.; Gingras, M.; Pasian, S.; Tsao, M-Sound.; Lam, S.; Sin, D.D.; Ionescu, D.; Atkar-Khattra, S.; Elwood, M.; Gee, S.; Cressman, S.; Sonnichsen, D.; McWilliams, A.; Puksa, S.; Evans, B.; Moore, E.; Couture, C.; St-Pierre, C.; Pennell, E.; Sekhon, H.; Amjadi, K., 2016: Plasma pro-surfactant protein B and lung function decline in smokers. Plasma pro-surfactant protein B (pro-SFTPB) levels have recently been shown to predict the development of lung cancer in current and ex-smokers, but the ability of pro-SFTPB to predict measures of chronic obstructive pulmonary disease (COPD) sever...

Kenknight, Glenn, 1958: Thermal stability of peach rosette virus. Thermal death time of peach rosette virus (PRV) had been reported as about 10 minutes at 50[degree]C. Variable results from heat treatments at Fort Valley led to the development of the "nurse-tree" method whereby PRV thermal death time w...

Lenfant, Fçoise.; Trémollières, F.; Gourdy, P.; Arnal, J-François., 2011: Timing of the vascular actions of estrogens in experimental and human studies: why protective early, and not when delayed?. Estrogens, and in particular 17β-estradiol (E2), play a pivotal role in sexual development and reproduction and are also implicated in a large number of physiological processes including the cardiovascular system. Although epidemiological studies...

Tgavalekos, N.T.; Venegas, J.G.; Suki, B.; Lutchen, K.R., 2003: Relation between structure, function, and imaging in a three-dimensional model of the lung. Previous studies have reported morphometric models to predict function relations in the lung. These models, however, are not anatomically explicit. We have advanced a three-dimensional airway tree model to relate dynamic lung function to alteratio...