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Separation of selected metal-binding proteins with capillary zone electrophoresis

, : Separation of selected metal-binding proteins with capillary zone electrophoresis. Analytica Chimica Acta 477(1): 1-19, 27 January

Several metal-binding proteins, including albumin, carbonic anhydrase, conalbumin, cytochrome c, ferritin, hemoglobin, myoglobin, plasma amine oxidase, superoxide dismutase and transferrin were separated with capillary zone electrophoresis (CZE) in uncoated and coated capillaries. Phosphate and tetraborate buffers achieved complementary separation selectivities. Optimised pre-wash protocols for uncoated capillaries using 0.1 M HCl as a rinsing solution for the borate buffer and a combination of 0.1 M NaOH and 0.1 M HCl for the phosphate system improved the stability of migration times considerably with coefficients of variation between 0.10 and 0.77% (n=7) instead of up to 2.92% with inappropriate rinsing conditions. Capillaries coated with poly(vinyl alcohol) and equipped with a 150 mum i.d. bubble cell increased the signal-to-noise ratio by a factor three, additionally improving the resolution. For commercial protein standards, which gave several peaks in CZE with UV detection, MS data proved the presence of proteinaceous contaminants. Molecular weights (Mr) of proteins experimentally determined from MS data showed deviations from theoretical Mr as small as 0.002-0.021%. Applicability of the developed separation for clinical samples is shown for human serum.


DOI: 10.1016/s0003-2670(02)01403-4

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