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Serum IFN-gamma and IL-10 levels are associated with disease progression in non-obese diabetic mice

, : Serum IFN-gamma and IL-10 levels are associated with disease progression in non-obese diabetic mice. Diabetes/Metabolism Research and Reviews 18(1): 64-70

Background: The goal of the present study was to determine whether cytokines in the peripheral blood of naive NOD mice correlate with the disease process and thereby would provide a marker for monitoring disease activity. Methods: Female NOD mice (5, 10 and 14-16 weeks of age) were investigated in a cross-sectional study. In the group of 14-16-week-old mice, non-diabetic and diabetic mice were analysed as different subgroups. The Th1 cytokine (IFN-gamma) and the Th2 cytokine (IL-10) were quantified in serum by sandwich enzyme-linked immunosorbent assay (ELISA). Pancreatic mRNA for IFN-gamma and IL-10 was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) from the same animals. Results: Serum levels of IFN-gamma were initially low but increased with age in NOD mice, reaching the highest levels at diabetes onset (p<0.002 compared to 10 weeks). A similar rise was noted in IFN-gamma gene expression in pancreatic lesions. In contrast, an early peak of serum IL-10 levels was observed in non-diabetic NOD mice (10 weeks) at a stage where non-destructive insulitis occurs. With increasing age a continuous loss of IL-10 until progression towards diabetes was observed. The pancreatic IL-10 mRNA expression correlated with serum IL-10 changes. As a consequence, the ratio of IFN-gamma/IL-10, reflecting the Th1/Th2 balance in the serum, was significantly increased in diabetic compared to non-diabetic NOD mice (p<0.005). Conclusion: These results demonstrate, for the first time, that an increased Th2 pattern in the non-diabetic stage preceding a Th1 shift is associated with the development of diabetes in naive NOD mice. Serum cytokines correlate with disease progression and pancreatic cytokine expression during prediabetes. Soluble cytokines measured in the periphery are therefore promising surrogate markers of diabetes development.


PMID: 11921420

Other references

Foster, L.M., 1987: Orange County Farm Labor Program summary. American Journal of Public Health 77(7): 874-875

John S.B.imacombe; Annalee S.M.ngech; May S.S.eed, 1979: Some approaches to the synthesis of evernitrose and its enantiomer. Carbohydrate Research 75(none): 0-0

Romano, P.E., 1986: Pro steroids for systemic antifibrinolytic treatment for traumatic hyphema. Journal of Pediatric Ophthalmology and Strabismus 23(2): 92-95

Anonymous, 1970: New species since Hoffmeyer. Lepidoptera (new ser) 1 (10) 234-237

Yao, X.S.; Ebizuka, Y.; Noguchi, H.; Kiuchi, F.; Sankawa, U.; Seto, H., 1983: Structure of arnebinone, a novel monoterpenylbenzoquinone with inhibitory effect to prostaglandin biosynthesis. Arnebia euchroma roots yielded a pale yellow oil (identified as a mixture of shikonofuran B and C) and a quinone, the structure of which is described.

Lindemann, E., 1919: Untersuchungen uber Susswasserperidineen und ihre Variationsformen. Archiv fuer Protistenkunde Jena, 39 209-262

Lin, J.; Zhang, Y.-Lin; Wang, Y.-Lun, 2011: A comparative study on the female reproductive system of four species of the fulgoroidea hemiptera. The female structures of the genitalia and internal reproductive organs, which belong to Salurnis marginella (Guerin-Meneuille) (Flatidae), Ricania speculum (Walker) (Ricaniidae), Lycorma delicatula (Walker) (Fugoridae), Raivuna sinica (Walker) (D...

Zuyev V.N.; Zubkov L.B.; Chistov L.B., 1967: The forms of tantalum and niobium in wolframite-bearing greisenized granite and tungsten ores of the USSR. Columbite and microlite inclusions in muscovite and wolframite, tantaloniobate minerals, isomorphic replacement.

Harthorne, J.W.; Buckley, M.J.; Grover, J.W.; Austen, W.G., 1967: Valve replacement during pregnancy. The normal physiologic response to pregnancy includes a rise in the resting cardiac output of 30% to 50%, 30% increase in blood volume, 20% increase in O2 consumption, and an increase in the heart rate of 15%. in the pregnant female with underlyin...

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