geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Specificity determinants in the interaction of apolipoprotein(a) kringles with tetranectin and LDL


, : Specificity determinants in the interaction of apolipoprotein(a) kringles with tetranectin and LDL. Biological Chemistry 383(11): 1743-1750

Lipoprotein(a) is composed of low density lipoprotein and apolipoprotein(a). Apolipoprotein(a) has evolved from plasminogen and contains 10 different plasminogen kringle 4 homologous domains (KIV(1-110)). Previous studies indicated that lipoprotein(a) non-covalently binds the N-terminal region of lipoprotein B100 and the plasminogen kringle 4 binding plasma protein tetranectin. In this study recombinant KIV(2), KIV(7) and KIV(10) derived from apolipoprotein(a) were produced in E. coli and the binding to tetranectin and low density lipoprotein was examined. Only KIV(10) bound to tetranectin and binding was similar to that of plasminogen kringle 4 to tetranectin. Only KIV(7) bound to LDL. In order to identify the residues responsible for the difference in specificity between KIV(7) and KIV(10), a number of surface-exposed residues located around the lysine binding clefts were exchanged. Ligand binding analysis of these derivatives showed that Y62, and to a minor extent W32 and E56, of KIV(7) are important for LDL binding to KIV(7), whereas R32 and D56 of KIV(10) are required for tetranectin binding of KIV(10).

US$19.90

PMID: 12530539

DOI: 10.1515/BC.2002.195


Other references

Blount, J.F.; Mohacsi, E.; Vane, F.M.; Mannering, G.J., 1973: Isolation, x-ray analysis, and synthesis of a metabolite of (-)-3-hydroxy-N-allylmorphinan. Journal of Medicinal Chemistry 16(4): 352-355

Farah, A., 1952: Renal vascular changes produced by the mercurial diuretic salyrgan. Nembutalized dogs were infused with isotonic, hypertonic or hypotonic saline to which adequate amts. of creatinine or inulin and p-aminohippuric acid were added. Varying amts. of salyrgan were given intraven. Blood pressure, renal blood flow, glom...

Khare, S.; Bissonnette, M.; Roy, H.; Suh, W.; Sitrin, M.D.; Brasitus, T.A., 2000: 1,25-dihydroxyvitamin D3 stimulates the Ras, MEK, ERK signaling pathway in rat colonocytes. Gastroenterology 118(4 Suppl 2 Part 1): A89, April

Jensen, C.J.P.; Adams, G.C., 1995: Nitrogen metabolism of Leucostoma persoonii and L. cincta in virulent and hypovirulent isolates. The nitrogen utilization pattern of 2 species of Leucostoma was examined using virulent isolates of L. persoonii and L. cincta [L. cinctum], and 2 virus-infected hypovirulent isolates of L. persoonii. Virulent L. persoonii were able to utilize all...

Roth, G.A.; Gonzalez, M.D.; Monferran, C.G.; D.S.ntis, M.L.; Cumar, F.A., 1993: Myelin basic protein domains involved in the interaction with actin. A fluorescence assay was used to measure the interaction of myelin basic protein (MBP) with monomeric actin labeled with a fluorescent compound (IAEDANS). The complex actin-IAEDANS increase the fluorescence in presence of MBP. The enhancement of t...

Harnadek, M.C.S.; Martzke, J.S.; Grace, G.M., 1993: Serial neuropsychological evaluation of a patient following prolonged anoxia. Journal of Clinical & Experimental Neuropsychology 15(1): 106

Bezkorovainaia, Z.G., 1958: Study of the mechanism of the positive action of water-soluble vitamins on liver function in various subjects. Trudy Leningradskogo Sanitarno-Gigienicheskogo Meditsinskogo Instituta 50: 36-49

Marchal, G.J.; Desmet, V.J.; Proesmans, W.C.; Moerman, P.L.; Van Roost, W.W.; Van Holsbeeck, M.T.; Baert, A.L., 1986: Caroli disease: high-frequency US and pathologic findings. Three infants with nonobstructive bile duct dilatation (Caroli disease) are described. The bile duct pathology was associated with autosomal recessive polycystic kidney disease in two patients and with severe destruction of the renal parenchyma of...

Music, S.I.; Libonati, J.P.; Wenzel, R.P.; Snyder, M.J.; Hornick, R.B.; Woodward, T.E., 1970: Induced human cholera. Antimicrobial Agents and ChemoTherapy 10: 462-466

Kim, E-Ah.; Lawler, M.; Vishveshwara, S.; Fradkin, E., 2005: Signatures of fractional statistics in noise experiments in quantum Hall fluids. The elementary excitations of fractional quantum Hall (FQH) fluids are vortices with fractional statistics. Yet, this fundamental prediction has remained an open experimental challenge. Here we show that the cross-current noise in a three-terminal...