geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Stereoselective synthesis of (S)-(+)-Naproxen catalyzed by carboxyl esterase in a multicompartment electrolyzer


, : Stereoselective synthesis of (S)-(+)-Naproxen catalyzed by carboxyl esterase in a multicompartment electrolyzer. Journal of Biochemical and Biophysical Methods 48(3): 247-256

The stereoselective hydrolysis of racemic 2-substituted propionates, catalyzed by carboxyl esterase, provides a cost-competitive route to produce the optically pure, anti-inflammatory drug Naproxen. In the present work, we describe the application of the multicompartment electrolyzer reactor (ME) for the stereoselective hydrolysis of a racemic Naproxen ester, (R,S)-ethoxyethyl-[2-(6-methoxy-2-naphtyl)]propionate, catalyzed by a carboxyl esterase. The enzyme was trapped in a reactor chamber, delimited by two isoelectric membranes encompassing the pI value of the enzyme, together with the neutral substrate. After 90 min, a conversion of 45% was obtained with an enantiomeric excess of 84%. The reaction product, (S)-(+)-Naproxen, was electrophoretically removed in continuous from the reaction chamber and collected in a contiguous, more acidic chamber, separated from the enzyme and from the unreacted substrate. Moreover, at the end of the reaction, it was possible to recover the enzyme from the reactor and use it again.

US$19.90

PMID: 11384761

DOI: 10.1016/s0165-022x(01)00158-0


Other references

Elliott, J.; Bray, G., 1979: George Bray: the nutrition message must be spread. JAMA 241(13): 1320-1321

Sun J.Zhou, 2000: Appraisal on the influence upon the Jiu Dian Xia key water control project of the Taohe river to plants and vegetation in reservoir region. Through an investigation on the conditions of plant and vegetation of reservoir region and analyses of factor of influence upon the Jiu Dian Xia key water control project of the Taohe river to plants and vegetation in reservoir region, this paper...

Duan, X.; Ponomareva, L.; Veeranki, S.; Panchanathan, R.; Dickerson, E.; Choubey, D., 2011: Differential roles for the interferon-inducible IFI16 and AIM2 innate immune sensors for cytosolic DNA in cellular senescence of human fibroblasts. The IFN-inducible IFI16 and AIM2 proteins act as innate immune sensors for cytosolic double-stranded DNA (dsDNA). On sensing dsDNA, the IFI16 protein induces the expression of IFN-β whereas the AIM2 protein forms an inflammasome, which promot...

Novak F.; Takac P.; Lambert J.; D.L.of A., 1989: Identification and concentrations of nonecdysteroid steroids in a few insect species. General & Comparative Endocrinology 74(2): 260

Paramonov, S.V.; Lokshin, V.; Ihmels, H.; Fedorova, O.A., 2011: Influence of DNA-binding on the photochromic equilibrium of a chromene derivative. The photochromic properties of a chromene derivative, which contains a positively charged substituent, were investigated in the presence of ct DNA. Upon irradiation in the absence of DNA the colored quinoid open form is highly unstable and rapidly...

Ritzefeld, M.; Sewald, N., 2012: Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Several proteins, like transcription factors, bind to certain DNA sequences, thereby regulating biochemical pathways that determine the fate of the corresponding cell. Due to these key positions, it is indispensable to analyze protein-DNA interact...

Johannsen, U., 1974: Pathology and pathogenesis of spontaneous coli enterotoxaemia and the experimental coli endotoxin syndrome in pigs. VII. Pathology of the mesenteric nodes. Histological examination of mesenteric lymph nodes from 71 cases of spontaneous coli enterotoxaemia (CE) and 28 cases of experimental coli endotoxin shock (ES) showed that CE was not accompanied by any typical, recurrent and constant changes. The...

Morvan, G., 1989: Research work and policy of control against sharka. Sharka, caused by plum pox potyvirus, is a severe threat to the apricot industry in the south-east of France. Although it was introduced 27 years ago, it affects a limited number of trees. This was achieved due to an active policy of sanitation of...

Pollington, S., 2012: Novel glass-ceramics for dental restorations. There are many different ceramic systems available on the market for dental restorations. Glass-ceramics are a popular choice due to their excellent esthetics and ability to bond to tooth structure allowing a more conservative approach. However, a...

Claussen D.L., 1972: Urinary bladder water reserves in the terrestrial toad bufo fowleri and the aquatic frog rana clamitans. American Zoologist 12(4): 679-680