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Structure of d(CGCGAATTCGCG) in the presence of Ca(2+) ions


, : Structure of d(CGCGAATTCGCG) in the presence of Ca(2+) ions. Journal of Biological Chemistry 274(35): 24749-24752

The dodecamer d(CGCGAATTCGCG) was the first oligonucleotide to be crystallized as a B-DNA duplex. Its structure was analyzed in detail in the early 1980s. Here we show that, in the presence of Ca2+, it crystallizes in a different way (R3 space group). The dodecamers form parallel columns of straight duplexes with ten base pairs in the B form. The terminal cytosines in each molecule are disordered, whereas the terminal guanines are placed in the minor groove of neighbor duplexes.

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PMID: 10455145


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