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Studies of lectin receptors of rat microglia in culture: Receptor distribution and internalization


, : Studies of lectin receptors of rat microglia in culture: Receptor distribution and internalization. Experimental Brain Research 124(1): 89-99, Jan

The present study examined the lectin labeling of diverse morphological forms of microglia in culture. Similar to amoeboid microglial cells in vivo, polymorphic microglia showed lectin labeling at their plasma membranes, as well as in a few cytoplasmic vesicles and vacuoles. This labeling pattern was observed in cultured microglia incubated with isolectin at 4 degrees C for 30 min. Five minutes after the temperature was raised to 37 degrees C, the surface lectin receptors appeared to be internalized, as shown by the occurrence of many subsurface lectin-labeled vesicles, vacuoles and tubule-like structures. With longer incubation (up to 1-2 h at 37 degrees C), many lysosomes and a few trans-Golgi saccules and associated lysosome-like structures became labeled. Concomitant with these changes was a reduction of lectin labeling at the plasma, with labeling having vanished in most of the cells after 1-2 h of incubation. By 24 h, only a few cells retained surface lectin labeling. It appears, therefore, that irrespective of morphology, lectin labeling (including its intracellular pathway) of microglia in culture parallels that of amoeboid microglia in vivo. This would offer a useful model for the study of lectin turnover in microglia and help to explain the roles of such receptors in microglial differentiation and function.

US$19.90

PMID: 9928793

DOI: 10.1007/s002210050603


Other references

Ghiglieri, V.; Picconi, B.; Calabresi, P., 2010: Direct and indirect pathways in levodopa-induced dyskinesia: a more complex matter than a network imbalance. Movement Disorders 25(11): 1527-1529

Fekecsová, S.; Danchenko, M.; Uvackova, L.; Skultety, L.; Hajduch, M., 2015: Using 7 cm immobilized pH gradient strips to determine levels of clinically relevant proteins in wheat grain extracts. The aim of the work was to test a relatively simple proteomics approach based on phenol extraction and two-dimensional gel electrophoresis (2-DE) with 7 cm immobilized pH gradient strips for the determination of clinically relevant proteins in whe...

Kolesnikov, S.A.; Ivanitskaya, M.A.; Petrosyan, Y.S.; Tsukerman, G.I., 1965: Possibilities of making a clinical diagnosis of cardiac myxoma. Until today diagnosis of heart myxoma during life is a rarity. However, this disease has signs which permit its diagnosis. Patients with such signs must be examined cardiologically including angiocardiography. If diagnosis of heart myxoma is confi...

Gill, S.; Kauffman, E.C.; Kandel, S.; George, S.; Schwaab, T.; Xu, B., 2015: Incidence of Clear Cell Papillary Renal Cell Carcinoma in Low-Grade Renal Cell Carcinoma Cases: A 12-Year Retrospective Clinicopathologic Study From a Single Cancer Center. Clear cell papillary renal cell carcinoma (CCPRCC) is a recently recognized subtype of renal cell carcinoma entity after 2004 World Health Organization classification of renal tumors. CCPRCC has unique histomorphological and immunohistochemical ch...

Raben, A.S.; Matseĭna, S.; Lorie, I.I., 1970: Case of secondary recurring syphilis erroneously diagnosed as lymphogranulomatosis. Klinicheskaia Meditsina 48(10): 97-99

Liu, H.; Ren, G.; Zhu, L.; Liu, X.; He, X., 2015: The upregulation of miRNA-146a inhibited biological behaviors of ESCC through inhibition of IRS2. In recent years, microRNAs, also called as miRNAs, play an important role in carcinogenesis, and the dysregulation of miRNAs is closely associated with cancer progression. Till now, little has been known about the role of miRNA-146a in the esophag...

Kerns, J.M.; Pavkovic, I.M.; Fakhouri, A.J.; Gray, G.T., 1994: Electrical stimulation of nerve regeneration in the rat: functional evaluation by a twitch tension method. The effect of D.C. electrical fields on in vivo nerve regeneration following rat sciatic nerve transection was assessed by a toe-twitch tension method for motor recovery. Two groups for baseline comparisons received either a nerve transection and...

Lu, C.F.; Springer, T.A., 1996: The alpha subunit cytoplasmic domain regulates adhesive activity and alpha-beta subunit dimerization of LFA-1. FASEB Journal 10(6): A1228

Elmslie, R.C., 1924: Case of Snapping Knee. Proceedings of the Royal Society of Medicine 17(Sect Orthop): 15-15

Kasai, M.; Oosawa, F., 1963: Removal Of Nucleotides From F-Actin. Biochimica et Biophysica Acta 75: 223-233