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Study on M genomic segment of hantavirus strains isolated from different areas in Hubei province

, : Study on M genomic segment of hantavirus strains isolated from different areas in Hubei province. Virologica Sinica 17(1): 26-29, February

According to Genebank sofeware of HV, the type-specific primers were desisgned correspording to the conserved sequences in the G1 region of M genomic segment of HV. A reverse-transcription heminested polymerase chain reaction for HV was developed. Then, RT-heminested PCR was applied to type 30 strains of HV isolated from different areas in Hubei province. The results are shown that: (1) The 76/118, A9, H-114 (HTNV) and R22 (SEOV) Strains of HV were amplified and typed using the RT-heminested PCR. And HV RNA could only be detected by this method, but other viral RNA couldn't be detected. (2) 30 strains isolated from different areas in Hubei province were typed as HTNV amd SEOV, respectively. Those results suggested that: (1) The RT-heminested PCR was feasible for detectiong and typing HV in Hubei province. (2) Both HINV and SEOV were found in epidmic areas of HFRS in Hubei province. Distinct hantaviruses could coexist in either different or the same geographic or ecological zones in Hubei province.


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