geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

TRPC3-like protein is involved in the capacitative cation entry induced by 1alpha,25-dihydroxy-vitamin D3 in ROS 17/2.8 osteoblastic cells


, : TRPC3-like protein is involved in the capacitative cation entry induced by 1alpha,25-dihydroxy-vitamin D3 in ROS 17/2.8 osteoblastic cells. Journal of Cellular Biochemistry 90(1): 197-205

In ROS 17/2.8 rat osteoblastic-like cells a capacitative Ca2+ entry (CCE) pathway operates which is activated by either 1alpha,25-dihydroxy-vitamin D3 (1alpha,25(OH)2D3) or thapsigargin (Tpg)-induced depletion of Ca2+ stores (Baldi et al. (2002): J. Cell. Biochem. 86:678-687). In view of recent evidence favoring a role for transient receptor potential (TRP) proteins in mediating CCE, we investigated if channels involved in the 1alpha,25(OH)2D3-sensitive CCE in rat osteoblasts were related to an endogenous TRP-canonical (TRPC) isoform homologue. By reverse transcription (RT)-PCR using mRNA from ROS 17/2.8 cells and primers based on conserved regions within the mammalian TRPC3/6/7 subfamily, two fragments were amplified of 390 and 201 bp with 100 and 94% sequence identity, respectively, with human TRPC3. Northern blot analysis showed the presence of a 3.5 kb transcript and both immunobloting and immunocytochemistry using a specific anti-TRPC3 antibody confirmed endogenous expression of a TRPC3-like protein (apprx110 kDa) with membrane localization. In ROS 17/2.8 cells intranuclearly microinjected with anti-TRPC3 antisense oligodeoxynucleotides (ODN), both the initial rate and magnitude of CCE activated by either 1alpha,25(OH)2D3 or Tpg were markedly reduced, whereas no changes were detected in control-injected cells. The present findings constitute the first evidence to date suggesting that an endogenous TRPC3-like protein is functionally involved in the CCE route activated by 1alpha,25(OH)2D3 in a secosteroid target cell. We anticipate TRPC3 as a candidate for mediating store-operated non-selective cation entry into osteoblasts.

US$19.90

PMID: 12938168

DOI: 10.1002/jcb.10612


Other references

Torrent, M.; Isabel Geli, M.; Ludevid, M.D., 1989: Storage-protein hydrolysis and protein-body breakdown in germinatedZea mays L. seeds. Storage proteins of maize (Zea mays L.) were studied in germinated seeds, as were the proteins of protein bodies isolated from endosperms at different germination times. Major endosperm storage proteins were degraded in a sequential way, glutelin...

Marcus, J.; Elkins, G.; Mott, F., 2003: A model of hypnotic intervention for palliative care. The World Health Organization defines palliative care as "the active total care of patients whose disease is not responsive to curative treatment." One of the primary issues of palliative care for patients with advanced cancer is symptom...

Schack, B.; Krause, W., 1995: Dynamic power and coherence analysis of ultra short-term cognitive processes--a methodical study. This EEG mapping study was designed to explore ultra short-term cognitive processes in human thinking. The EEG was recorded while the subjects performed two tasks randomly mixed in time: category concept activation and pattern comparison. A novel...

Lee, E.H.; Hsin, J.; Mayans, O.; Schulten, K., 2007: Secondary and tertiary structure elasticity of titin Z1Z2 and a titin chain model. The giant protein titin, which is responsible for passive elasticity in muscle fibers, is built from approximately 300 regular immunoglobulin-like (Ig) domains and FN-III repeats. While the soft elasticity derived from its entropic regions, as wel...

Alford, C., 1986: Husband and wife team. Interview by Laurence Dopson. Nursing Times 82(18): 60-60

Yao S K.; Mcnatt J.; Benedict C.R.; Rosolowsky M.; Anderson H.V.; Cui K.; Maffrand J P.; Buja L.M.; Willerson J.T., 1991: Clopidogrel prevents platelet aggregation and cyclic flow variations in stenosed and endothelium injured canine coronary arteries. Journal of the American College of Cardiology 17(2 SUPPL A): 299A

Tacca, O.; Penault-Llorca, F.; Abrial, C.; Raoelfils, I.; Mouret-Reynier, M.A.; Bougnoux, P.; Achard, J.L.; Dauplat, J.; Chollet, P.; Curé, H., 2016: Neoadjuvant chemotherapy (NCT) in 710 patients for operable breast cancer: Variation changing in hormonal receptors (HR) status. NlmCategory="UNASSIGNED">673 Background: Positive HR constitutes by their presence a favourable prognosis factor and predicts response to an adjuvant hormonal therapy. In breast cancer treated by NCT, HR expression has been shown to b...

Czekaj, H.; Samorek-Salamonowicz, E.; Kozdrun, W., 2002: Charakterystyka szczepu reowirusa wyizolowanego z przypadku reowirozy brojlerow. Investigations were carried out on chickens coming from parent flocks vaccinated against reoviruses. Beginning from the seventh day of life, difficulties in moving, leg paralysis, decreased appetite and sporadic diarrhoea were observed in these ch...

Higgins, T.F.; Kemper, D.; Klatt, J., 2009: Incidence and morphology of the posteromedial fragment in bicondylar tibial plateau fractures. Bicondylar tibial plateau fractures featuring extensive articular involvement have a record of mixed clinical results. Recent discussion has focused on the significance of a posteromedial articular fragment in bicondylar injuries. This fragment ha...

Chatin, J., 1896: De la phagocytose chez les Huitres. Compte Rendu de l'Academie des Sciences Paris, cxxii 487-490