+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Testicular sperm extraction by Trucut needle and milking of seminiferous tubules: A technique with high yield and patient acceptability

, : Testicular sperm extraction by Trucut needle and milking of seminiferous tubules: A technique with high yield and patient acceptability. Fertility & Sterility 74(2): 380-383, August

Objective: To assess the sperm yield and patient acceptability of Trucut needle testicular biopsy followed by seminiferous tubule milking. Design: Prospective case analysis. Setting: The Regional Fertility Center, Royal Maternity Hospital, Belfast, Northern Ireland, United Kingdom. Patient(s): Forty-one males with obstructive azoospermia (normal testicular volume and FSH and LH levels). Intervention(s): Trucut needle testicular biopsies under local anesthetic with milking of the seminiferous tubules. Main Outcome Measure(s): Quantitation of sperm retrieved per biopsy core and patient follow-up by questionnaire. Result(s): A mean of 105,634 sperm (range, 5,000-427,800) were retrieved, and the mean biopsy weight was 9.17 mg. Twenty-six subjects found the biopsy painless and 15 were pain-free after biopsy. Conclusion(s): The Trucut needle can be used in combination with seminiferous tubule milking to obtain large numbers of sperm in men with obstructive azoospermia.


PMID: 10927063

DOI: 10.1016/s0015-0282(00)00655-5

Other references

Douglas, R.H.; Hultquist, D.E., 1978: Evidence that two forms of bovine erythrocyte cytochrome b5 are identical to segments of microsomal cytochrome b5. Homogeneous preparations of two forms of soluble cytochrome b5 have been obtained from bovine erythrocytes by successive chromatography on DEAE-cellulose, Bio-Gel P-60, and DEAE-Sephadex. Although the two forms could be separated on disc gel elect...

Ferré, S.; Karcz-Kubicha, M.; Hope, B.T.; Popoli, P.; Burgueño, J.; Gutiérrez, M.Angeles.; Casadó, V.; Fuxe, K.; Goldberg, S.R.; Lluis, C.; Franco, R.; Ciruela, F., 2002: Synergistic interaction between adenosine A2A and glutamate mGlu5 receptors: implications for striatal neuronal function. The physiological meaning of the coexpression of adenosine A2A receptors and group I metabotropic glutamate receptors in gamma- aminobutyric acid (GABA)ergic striatal neurons is intriguing. Here we provide in vitro and in vivo evidence for a syner...

Zilişteanu, C.; Mintzer-Morgenstern, L.; Ionesco, H.; Ionesco-Dorohoi, T., 1969: Further data on the association of bacteriophage with antibiotic therapy for the purpose of sterilizing carriers of dysentery bacilli. Archives Roumaines de Pathologie Experimentales et de Microbiologie 28(4): 1073-1080

Hammond, A.L., 1972: Mars as an active planet: the view from mariner 9. The current crisis in American archeology has been brought about by a combination of the greatly increased rate of destruction of unique, irreplaceable archeological information and material, and the lack of adequate funding for salvage of what is...

Anonymous, 2012: Variations in Diet and Stature, Are They Linked? Bioarchaeology and Paleodietary Bayesian Mixing Models from Linkoping, Sweden. From the early 19th century to the late 2th century, the mean stature of Swedish conscripts increased from 167 to 177?cm. Based on osteological data, estimated statures of medieval skeletal populations indicate that mean stature of males had decre...

Anner, B.M., 1985: The receptor function of the Na+, K+-activated adenosine triphosphatase system. Biochemical Journal 227(1): 1-11

Levi, M.I.; Ulukhanov, C.B.; Kuznetsova, K.A., 1989: Detection of plague microbes in fleas using immunoenzyme technics and monoclonal antibodies. The paper presents the results of immunoassays for indication of pest causative agents in fleas, this method being compared to the classical and bacteriological methods. Experiments proved the feasibility of indication of pest causative agents in...

Charan N.B.; Lakshminarayan S.; Albert R.K.; Butler J., 1981: Effect of prostaglandin inhibitors on systemic hypoxemia induced and hypercarbia induced increases in bronchial blood flow. American Review of Respiratory Disease 123(4 PART 2): 195

Bierhoff, M.L.; Levine, G.M., 1988: Luminal and metabolic regulation of jejunal amino acid absorption in the rat. Studies were carried out to determine the role of luminal amino acids and metabolic balance in in vivo amino acid absorption. Previous in vitro studies have shown that adaptation of amino acid transport is a complex phenomenon. In the first series...

Cantelmi, A.; Gaiti, A.; Porcellati, G.; Ansidei, V.; Binazzi, M., 1982: Modulation of phospholipase A activity in psoriatic skin. The activity of phospholipase A2 was tested both in rat skin and human psoriatic skin before and after systemic treatment with a retinoic acid derivative: RO 10-9359. An increase of activity was found in the rat skin homogenates when the animals w...