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Testing the SCE mechanism with non-poisoning topoisomerase II inhibitors


, : Testing the SCE mechanism with non-poisoning topoisomerase II inhibitors. Mutation Research 497(1-2): 71-79, 18 October

There are controversial theoretical models about a possible involvement of DNA topoisomerase II (topo II) in the molecular mechanism of sister chromatid exchanges (SCEs). In order to clarify the role of this enzyme, if any, in such recombinational event, CHO parental AA8 and mutant EM9 cells, which shows and extremely high baseline frequency of SCE, have been treated with different doses of the non-poisoning topoisomerase inhibitors, ICRF-193 and bufalin. The frequencies of SCEs after the treatments have been determined and the inhibitory effect of these compounds has been assessed using a topo II activity assay. The results indicate that ICRF-193 and bufalin effectively inhibit topo II activity in AA8 and EM9 cell lines. ICRF-193 induced a moderate increase in the frequency of SCEs in both types of cells, while bufalin did not modify the level of SCEs in any of them. The results are discussed taking into account the apparently unlike mechanisms of inhibition of topo II by ICRF-193 and bufalin.

US$19.90

PMID: 11525909

DOI: 10.1016/s1383-5718(01)00241-8


Other references

Kipfer, B.; Englberger, L.; Beer, J.H.; Lanz, M.; Carrel, T.P., 2000: Has lowered systemic heparinization during cardiopulmonary bypass a deleterious effect on platelet surface receptor GPIB. International Journal of Artificial Organs 23(8): 578, August

Hunault, G., 1973: Influence of different concentrations of 2,4-dichlorophenoxy acetic acid and benzyladenine on the growth of asparagus tissues (Asparagus officinalis L.) cultivated in vitro. Comptes rendus hebdomadaires des seances Serie D Sciences naturelles: 3, 296 (24) 3135-3138

Souckin, M.J., 1934: Infectious Metritis in Rabbits Corynebacterium Infection. This disease may be enzootic or sporadic. Generally there is great lassitude and hyperaemia and swelling of the vulva and vagina, with a blood-stained discharge; 60 per cent, of the infected animals may die, and abortion occurs in pregnant survivo...

Kuwayama, H., 1996: When should endoscopy be performed in patients with dyspepsia?. American Journal of Gastroenterology 91(10): 2251-2251

Oliveira, A.S.; Silva, P.B.; Lund, M.E.; Kersting, U.G.; Farina, D., 2013: Fast changes in direction during human locomotion are executed by impulsive activation of motor modules. This study investigated the modular control of complex locomotor tasks that require fast changes in direction, i.e., cutting manoeuvres. It was hypothesized that such tasks are accomplished by an impulsive (burst-like) activation of a few motor mo...

Newnham, W., 1849: Case of Abscess in the Spleen: Opening through the Left Lung above the Clavicle. Provincial Medical & Surgical Journal 13(15): 400-401

Riddet, W.; Neill, J.C., 1936: Butter-boxes and mould-growth. Experiments carried out in New Zealand showed that moulds (Pullularia pullulans, Cladosporium herbarum, Penicillium expansum, Mucor sp., and a red-staining type) originating in butter boxes may infect the surface layer of butter packed therein, an...

Ricordeau, G.; Passelecq, J., 1961: Anesthesia and reanimation for surgery of the adrenal. Anesthesie, Analgesie, Reanimation 18: 13-23

Sakai, H.; Tomino, Y.; Makino, H.; Arakawa, M.; Kurokawa, K.; Haneda, M.; Moriya, T.; Inomata, S.; Katayama, S.; Iwamoto, Y., 2002: A study about normal values of urinary type IV collagen concentration--aim at constructing a diagnostic guideline for diabetic nephropathy. Nihon Jinzo Gakkai Shi 44(5): 427-431

Jansen, E.F.; Curl, A.L.urence; Balls, A. . K., 1951: A crystalline, active oxidation product of or-chymotrypsin. alpha-Chymotrypsin when oxidized with Na periodate in the molar ratio of 5 to 20 moles of periodate per mole of enzyme resulted in the formation of an altered enzyme which was isolated in crystalline form. Non-protein N was formed during the oxida...