+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The Rho/Rho-kinase and the phosphatidylinositol 3-kinase pathways are essential for spontaneous locomotion of Walker 256 carcinosarcoma cells

, : The Rho/Rho-kinase and the phosphatidylinositol 3-kinase pathways are essential for spontaneous locomotion of Walker 256 carcinosarcoma cells. International Journal of Cancer 91(6): 763-771, 15 March

Signal transduction pathways controlling spontaneous locomotion of Walker carcinosarcoma cells are not well understood. We have therefore investigated the role of signalling proteins in development of polarity and locomotion of these cells. Treatment of the cells with 100 ng/ml pertussis toxin had no significant effect on the percentage of polarized cells. In contrast, 2 different phosphatidylinositol 3-kinase inhibitors (wortmannin and LY-294002) markedly reduced the proportion of polarized cells. Spontaneous locomotion of the cells was also significantly inhibited by these two inhibitors. In agreement with these data, we observed localization of the p85alpha subunit of phosphatidylinositol 3-kinase predominantly in the membrane fraction of Walker carcinosarcoma cells, indicating constitutive activation of this enzyme. We also investigated a role of Rho family proteins. Spontaneous development of polarity was almost completely suppressed by electroporation of the cells in the presence of 4 microg/ml C(3) exoenzyme, which specifically ADP-ribosylates and inactivates Rho. Two downstream targets of Rho, the Rho-activated kinases I and II, were also detected predominantly in the particulate membrane fraction, suggesting constitutive activation. A specific Rho-kinase inhibitor (Y-27632) blocked spontaneous polarization and migration in a concentration-dependent manner. Our results indicate that constitutive activation of the Rho/Rho-kinase pathway and of phosphatidylinositol 3-kinase plays an essential part in spontaneous development of polarity and cell locomotion of these cells.


PMID: 11275977

DOI: 10.1002/1097-0215(200102)9999:9999<::aid-ijc1128>;2-b

Other references

Müller, M.K.; Tremer, M.; Bodenstein, C.; Würtz, R.P., 2013: Learning invariant face recognition from examples. Autonomous learning is demonstrated by living beings that learn visual invariances during their visual experience. Standard neural network models do not show this sort of learning. On the example of face recognition in different situations we prop...

Kyazimov, IB., 1975: The influence of predators on the numbers of young salmon. Rybnoe Khozyaistvo Moskva, 19734: 18-19

Fornes, F.; Mendoza-Hernández, D.; García-de-la-Fuente, R.; Abad, M.; Belda, R.M., 2012: Composting versus vermicomposting: a comparative study of organic matter evolution through straight and combined processes. Changes in physical and chemical characteristics of an organic waste (tomato crop waste) throughout composting (COM), vermicomposting (VER) and the combination of both processes (C+V) were assessed at five selected stages. COM consisted of a combi...

Anonymous, 1996: Meetings calendar: meetings calendar. Environmental Science & Technology 30(5): 219a-219a, T.; Renner, M.F.rret, 2005: Contribution to the knowledge of Apostolepis flavotorquata Serpentes, Elapomorphinae Contribuicao ao conhecimento taxonomico de Apostolepis flavotorquata Serpentes, Elapomorphinae. The examination of the holotype, and more than one hundred specimens of Apostolepisflavotorquata (Dum6ril, Bibron and Dumdril, 1854), furnish many data on this bad knowledge species, and allows to redescribe it, with the description of the hernipe...

Sauter, T.C.; Lindner, G.; Ahmad, S.S.; Leichtle, A.Benedikt.; Fiedler, G-Martin.; Exadaktylos, A.K.; Haider, D.G., 2016: Calcium Disorders in the Emergency Department: Independent Risk Factors for Mortality. Calcium disorders are common in both intensive care units and in patients with chronic kidney disease and are associated with increased morbidity and mortality. It is unknown whether calcium abnormalities in unselected emergency department admissi...

Baron, F.; Legent, F., 1970: Post-traumatic perilymphatic fistula. Annales D'oto-Laryngologie et de Chirurgie Cervico Faciale 87(3): 137-144

Anonymous, 2011: Specific Nitration of Tyrosines 46 and 48 Makes CytochromecAssemble a Non-functional Apoptosome. Under nitroxidative stress, a minor fraction of cytochromeccan be modified by tyrosine nitration. Here we analyze the specific effect of nitration of tyrosines 46 and 48 on the dual role of cytochromecin cell survival and cell death. Our findings...

Frederic, J.; Lennès, G.; Rongé-Collard, E.; Mouchette, R.; Hausman-Hagemeijer, A., 1967: Demonstration, by statistical analysis, of a small translocation in a patient with Stein-Leventhal syndrome. Journal de Genetique Humaine 16(1): 25-41

Sebestyen, Ej, 1969: Contribution to the understanding of dosing and mixing problems in mixed fodder production. Muhle 106 (11) 161-162