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The Saccharomyces recombination protein Tid1p is required for adaptation from G2/M arrest induced by a double-strand break


, : The Saccharomyces recombination protein Tid1p is required for adaptation from G2/M arrest induced by a double-strand break. Current Biology 11(13): 1053-1057, 10 July

Saccharomyces cells with a single unrepaired double-strand break (DSB) will adapt to checkpoint-mediated G2/M arrest and resume cell cycle progression. The decision to adapt is finely regulated by the extent of single-stranded DNA generated from a DSB. We show that cells lacking the recombination protein Tid1p are unable to adapt, but that this defect is distinct from any role in recombination. As with the adaptation-defective mutations yku70Delta and cdc5-ad, permanent arrest in tid1Delta is bypassed by the deletion of the checkpoint gene RAD9. Permanent arrest of tid1Delta cells is suppressed by the rfa1-t11 mutation in the ssDNA binding complex RPA, similar to yku70Delta, whereas the defect in cdc5-ad is not suppressed. Unlike yku70Delta, tid1Delta does not affect 5'-to-3' degradation of DSB ends. The tid1Delta defect cannot be complemented by overexpressing the homolog Rad54p, nor is it affected in rad51Delta tid1Delta, rad54Delta tid1Delta, or rad52Delta tid1Delta double mutants that prevent essentially all homologous recombination. We suggest that Tid1p participates in monitoring the extent of single-stranded DNA produced by resection of DNA ends in a fashion that is distinct from its role in recombination.

US$19.90

PMID: 11470411

DOI: 10.1016/s0960-9822(01)00296-2


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Swank, O.H., 2010: Why are junior doctors reluctant to consult attending physicians?. A physician performs two tasks: making diagnoses and determining treatments. To reduce medical error, junior doctors are supposed to consult their supervisors when they face uncommon circumstances. However, recent research shows that junior doctor...

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Cerbulis, J., 1969: Proteins extracted from whole milk chloroform-methanol (2:1) and with fifty percent aqueous ethanol. Milchwissenschaft 24 (3) 140-142

Kaguni, J.; LaVerne, L.S.; Ray, D.S., 1979: Cloning and expression of the Escherichia coli replication origin in a single-stranded DNA phage. The Escherichia coli DNA replication origin (oriC) and the adjacent asparagine synthetase gene (asnA) have been inserted into the duplex replicative form DNA of the single-stranded phage vector M13Goril. By in vitro recombination, the entire oriC...

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Gomir, T.S.Ndrucci, M.; Nicola, P.; Mussa, G.C., 1959: Plasma and erythrocyte volume and half-life of erythrocytes in congenital heart disease. Minerva Pediatrica 11(25-26): 703-703

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