geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The influence of naturally occurring heterophilic anti-immunoglobulin antibodies on direct measurement of serum proteins using sandwich ELISAs


, : The influence of naturally occurring heterophilic anti-immunoglobulin antibodies on direct measurement of serum proteins using sandwich ELISAs. Journal of Immunological Methods 235(1-2): 71-80, Feb 21

Sandwich ELISAs have become a widely used method for the quantitative detection of serum proteins. However, they can be biased by a variety of interfering substances. As reported recently, we observed false-positive levels of interferon (IFN)-alpha and -beta in up to 27% of sera from healthy blood donors using commercial ELISAs. We now demonstrate that two different groups of naturally occurring heterophilic antibodies (IgG-type) are responsible for these titers. Group I (representing 85% of positive samples) binds to the Fab region of IgG from goat, mouse, rat, horse, and bovidae (but not rabbit). Group II (15%) recognizes an epitope in the Fc region of mouse, horse, bovine, and rabbit (but not goat or rat) immunoglobulins. The antibodies did not crossreact with human IgG subclasses but contributed to false-positive IgG rheumatoid factor levels obtained using a commercially available ELISA. To investigate the susceptibility of assays to these artifacts, various combinations of capture and detection antibodies have been tested. On this basis, we defined the relative risks that standard ELISAs might be influenced by heterophilic anti-immunoglobulin antibodies. In general, assays that use monoclonal antibodies for both capture and detection are less susceptible than others which include at least one polyclonal antiserum. However, only systems utilizing rabbit F(ab')(2) fragments have been found to be immune to this interference.

US$19.90

PMID: 10675759

DOI: 10.1016/s0022-1759(99)00206-9


Other references

Woodliff, H.J., 1972: Leukaemia and allied disorders. 3. Clinical findings in 200 personal cases. Medical Journal of Australia 2(8): 408-413

Morrissey, M.V., 2007: Suffer no more in silence: challenging the myths of women's mental health in childbearing. This article will challenge some of the myths surrounding women's mental health in childbearing. Pregnancy and the aftermath are very often seen as a time of joy and happiness. Yet for a significant amount of women at least ten per cent child...

Shin, C-Nam.; Lach, H.W., 2014: Acculturation and Health of Korean American Adults. Increasing cultural diversity in the United States and significant health disparities among immigrant populations make acculturation an important concept to measure in health research. The purpose of this cross-sectional, descriptive study was to...

Konishi, M.; Sugiyama, M., 2003: Genetic analysis of adventitious root formation with a novel series of temperature-sensitive mutants of Arabidopsis thaliana. When cultured on media containing the plant growth regulator auxin, hypocotyl explants of Arabidopsis thaliana generate adventitious roots. As a first step to investigate the genetic basis of adventitious organogenesis in plants, we isolated nine...

Wright, S., 1978: Screech beetles. Nottinghamshire Trust for Nature Conservation Ltd Newsletter, : 7-8

Zeme, D.; Targetti, M.; Sassu, G., 1972: Analgesic action of subcutaneous heparin therapy of stenocardia. La Clinica Terapeutica 62(3): 225-238

Made, D.; Wujanz, G., 1995: Investigations on the stabilization of pig blood for haematological investigations. Blood samples of 13 sows were preserved with ACD stabiliser with adenine, ACD stabiliser A (DAB) and heparin respectively and were stored in a refrigerator at 4 degrees C over a period of 18 days. During this time the number of erythrocytes and le...

Sharma, P.; Giribet, G., 2006: A new Pettalus species (Opiliones, Cyphophthalmi, Pettalidae) from Sri Lanka with a discussion on the evolution of eyes in cyphophthalmi. A new species of Cyphophthalmi (Opiliones) belonging to the Sri Lankan genus Pettalus is described and illustrated. Characterization of male and female genitalia and SEM illustrations are included, representing the first Such analysis for the gen...

Stine, W.B.; Murphy, A.; Ladu, M.J.; Krafft, G.A., 2000: Amyloid beta 1-40 and 1-42 solubilization parameters and structural outcomes. Since the initial determination of the primary sequence of the amyloid beta peptide (Abeta), a significant effort has been made to test the biological activities of synthetic Abeta in vitro. The peptide length, how peptide is initially reconstitut...

Spoelstra S.F., 1979: Volatile fatty acids in anaerobically stored piggery wastes. Volatile fatty acids (VFA) were estimated in 30 samples of farm slurries. The total concentration of VFA in these samples ranged 4.0-27.6 g/l (average 11.8 g/l with standard deviation of 5.6 g/l). Laboratory experiments showed that VFA are produce...