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The polycomb group protein EZH2 is upregulated in proliferating, cultured human mantle cell lymphoma


, : The polycomb group protein EZH2 is upregulated in proliferating, cultured human mantle cell lymphoma. British Journal of Haematology 112(4): 950-958, March

Polycomb group (PcG) proteins are involved in the stable transmittance of the repressive state of their gene targets throughout the cell cycle. Mis-expression of PcG proteins can lead to proliferative defects and tumorigenesis. There are two separate multimeric PcG protein complexes: an EED-EZH2-containing complex and a BMI1-RING1-containing complex. In the normal human follicle mantle, both PcG complexes have mutually exclusive expression patterns. BMI1-RING1 is expressed, but EZH2-EED is not. Here, we studied the expression of both complexes in six cases of mantle cell lymphoma (MCL), which is derived from the follicle mantle. MCL cells can be cultured in vitro and stimulated to proliferation. We found that resting MCL cells expressed BMI1-RING1, but not EZH2-EED, like normal mantle cells. Proliferating MCL cells, however, showed strongly enhanced expression of EZH2. Also, BMI1 and RING1 continued to be expressed in proliferating MCL. This is the first demonstration that EZH2 expression can be upregulated in fresh lymphoma cells. To test whether the enhanced EZH2 expression was causal for the increased proliferation in MCL, we overexpressed EZH2 in two different cell lines. In the B cell-derived Ramos cell line, EZH2 overexpression caused an increase in the proliferation rate. This suggests a possible causal effect between EZH2 upregulation and increased proliferation in haematopoietic cells.

US$19.90

PMID: 11298590

DOI: 10.1046/j.1365-2141.2001.02641.x


Other references

Postawski, K.; Przadka-Rabaniuk, D.; Piersiak, T., 2013: 8-oxo-7,8-dihydroguanine level - the DNA oxidative stress marker - recognized by fluorescence image analysis in sporadic uterine adenocarcinomas in women. In the case of carcinogenesis in human endometrium no information exists on tissue concentration of 8-oxo-7,8-dihydroguanine, the DNA oxidative stress marker This was the main reason to undertake the investigation of this DNA modification in human...

Boutton T.W., 1991: Tracer studies with carbon 13 enriched substrates humans and large animals. Coleman, D C And B Fry (Ed ) Isotopic Techniques in Plant, Soil, And Aquatic Biology: Carbon Isotope Techniques Xii+274p Academic Press, Inc : San Diego, California, Usa; London, England, Uk Illus 219-242

Johansson, G.; Akesson, A.; Berglund, M.; Nermell, B.; Vahter, M., 2000: Validation with biological markers for food intake of a dietary assessment method used by Swedish women with three different dietary preferences. To validate a dietary assessment method, a 4-day food record together with a duplicate portion technique, with biological markers for food intake. Four days of duplicate portions were collected in parallel with food recording. A 24-h urine sample...

Elattar T.M.A.; Tira D.E.; Lin H.S., 1983: Inhibition of prostaglandins and 12 l hydroxy eicosatetraenoic acid formation by gingival tissue. Journal of Dental Research 62(SPEC ISSUE): 212

Schultis, K., 1962: Positive N balances after operations. One of 2 mixtures of amino acids with added sources of energy, vitamins and electrolytes to provide adequate nutrition, Aminofusin 850 or Aminofusin Standard, was given by infusion for 8 days to 15 patients after operations. Balances of N were neg...

Brennan, R.F.; Mason, M.G.; Walton, G.H., 2000: Effect of nitrogen fertilizer on the concentrations of oil and protein in canola (Brassica napus) seed. The oil and protein concentration response of canola (Brassica napus L.) to application of nitrogen (N), applied as urea (46%), was measured in 210 field plots done in 1994 and 1995 in south-western Australia (WA). The canola was grown on a range...

Sheps, D.M.; Hildebrand, K.A.; Boorman, R.S., 2004: Simple dislocations of the elbow: evaluation and treatment. Simple dislocations of the elbow or dislocations occurring without an associated fracture are common injuries. Evaluation of these injuries must include an assessment of the entire involved upper extremity and a complete neurovascular examination....

Bogo, B., 1978: A system of maize growing used in Baja. Mezhdunarodnyi sel'skokhoziaistvennyi zhurnal: ) 41-43

Edison, B.R.; Lee Pace, J.; Brophy, C.H.; Mueske, N.M.; Katzel, M.J.; Zaslow, T.L.; Wren, T.A.L., 2016: Biomechanical Deficits During Drop Jump In Young Athletes With Recent Anterior Cruciate Ligament Reconstruction: 2635 Board #158 June 3, 11: 00 AM - 12: 30 PM. Medicine and Science in Sports and Exercise 48(5 Suppl 1): 735-735

Anonymous, 1995: Gene therapy for AIDS in an animal experiment. Fortschritte der Medizin 113(29): 14-14