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The structure of ADP-ribose pyrophosphatase reveals the structural basis for the versatility of the Nudix family

, : The structure of ADP-ribose pyrophosphatase reveals the structural basis for the versatility of the Nudix family. Nature Structural Biology 8(5): 467-472

Regulation of cellular levels of ADP-ribose is important in preventing nonenzymatic ADP-ribosylation of proteins. The Escherichia coli ADP-ribose pyrophosphatase, a Nudix enzyme, catalyzes the hydrolysis of ADP-ribose to ribose-5-P and AMP, compounds that can be recycled as part of nucleotide metabolism. The structures of the apo enzyme, the active enzyme and the complex with ADP-ribose were determined to 1.9ANG, 2.7ANG and 2.3ANG, respectively. The structures reveal a symmetric homodimer with two equivalent catalytic sites, each formed by residues of both monomers, requiring dimerization through domain swapping for substrate recognition and catalytic activity. The structures also suggest a role for the residues conserved in each Nudix subfamily. The Nudix motif residues, folded as a loop-helix-loop tailored for pyrophosphate hydrolysis, compose the catalytic center; residues conferring substrate specificity occur in regions of the sequence removed from the Nudix motif. This segregation of catalytic and recognition roles provides versatility to the Nudix family.


PMID: 11323725

DOI: 10.1038/87647

Other references

Anonymous, 1974: The world food supply-demand model and projections of agricultural products for 1980, 1985. The model projects world demand and supply of the major field crops (wheat, rice, maize, barley, sorghum and soybeans) and related major livestock products (beef, pork, poultry meat and milk), on a medium-term basis (in and around 1980) and also o...

Jeffs, P.W.; Johns, N.; Johnson, D.B., 1976: Synthesis of 2 tritiated dl tyrosine and 2 tritiated tyramine. Lithiation of 4-benzyloxy-2-bromotoluene and subsequent quenching of the lithiated product affords 4-benzyloxytoluene-2-t [t = tritiated] which is oxidized directly to 4-benzyloxybenzaldehyde-2-t with ceric ammonium nitrate. Transformation of this...

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Walz, M.; Wolff, M.; Voss, N.; Zabel, H.; Magerl, A., 2010: Micellar crystallization with a hysteresis in temperature. We have investigated the phase diagram of the triblock copolymer P123 solved in water by viscosity measurements for different concentrations and temperatures. The structures of the different phases were identified by surface sensitive neutron diff...

Tacker, P., 1991: Drainage for wheat production. FSA 91 (1012)

Mandal, S.K.; Saw, S.P.; Kumar, B.; Purbey, S.K., 2004: Bioefficacy of some granular insecticides against Leucinodes orbonalis Guen in brinjal. A field experiment was conducted during the 1990 kharif season in Ranchi, Bihar, India, on aubergine cv. Pusa Purple Round to study the efficacy of phorate, ethoprophos, carbofuran and aldicarb, each at 1.0 kg a.i./ha, against Leucinodes orbonalis...

Matveyev A.K.; Stefanova Y.I., 1978: Oil-, gas-, and coal-bearing capabilities of the Pacific Ocean mobile belt and the Pacific Ocean; oil shale. Pages 195-199 1978

Lau, W.Y.; Lai, E.C.H., 2007: Liver surgery and transplantation in China: Progress and Challenges. From the limited but available information, we traced the history of developments of liver surgery and transplantation in China. Liver surgery first started in the late 1950s in China, and it soon flourished mainly because of the great demand in l...

Riquimaroux H., 1991: Behavioral functions of subareas in the auditory cortex of the mustached bat revealed by a focal reversible inactivation. Neuroscience Research Supplement (16): S133

Vogel, Rudi, F., 1996: Biotechnology of starter organisms for non-dairy lactic food fermentations (mini-review). Food fermentations constitute biotechnical processes which use the metabolic potential of intrinsically safe microorganisms. Industrial exploitation and today's large scale production relies on the deliberate application of selected starter o...