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Transformation of an oral bacterium via chromosomal integration of free DNA in the presence of human saliva

, : Transformation of an oral bacterium via chromosomal integration of free DNA in the presence of human saliva. FEMS Microbiology Letters 200(2): 163-167, 25 June

Transformation of Streptococcus gordonii DL1 by free DNA was studied in human saliva. Competent S. gordonii could be transformed in vitro with plasmid DNA that had been taken into the human mouth. Transformation also occurred with a plasmid that cannot replicate in S. gordonii, but that has a region of chromosomal homology, by integration into the bacterial chromosome, although linearised plasmid DNA gave no transformants. Linear chromosomal DNA fragments did however transform S. gordonii/Tn916 efficiently in saliva when regions of homology with the recipient chromosome flanked the marker gene. These findings are discussed in relation to the potential for acquisition of DNA sequences, including genetically modified DNA, by gut and oral bacteria.


PMID: 11425469

DOI: 10.1111/j.1574-6968.2001.tb10709.x

Other references

Garcia Rodriguez, M.J.; Paupardin, C.; Saussay, R., 1978: The formation of secretory tissue and the synthesis of anethole by fennel tissue cultures. Unorganized and organized tissue cultures were obtained from fennel seedling apices on amended Murashige and Skoog medium supplemented with auxin and benzyladenine (BA). The best development was obtained in the presence of 10-8 g/l IAA and 10-6 g/...

Grinshpun, O.Ia, 1960: Mechano-electrical transformer for pulse recording. Fiziol Zhur Sssr [Transl] 46(3): 419-421

Kluft, R.P., 1992: The use of hypnosis with dissociative disorders. The dissociative disorders are characterized by difficulties in the integration of memory and/or identity. Typically this is manifested by amnesia and either the development of alternate identities or an estrangement from one's own identity....

Drain, D.J.; Martin, D.D.; Mitchell, B.W.; Seymour, D.E.; Spring, F.S., 1949: 4-Aminosalicylic acid and its derivatives. The acid obtained by carboxylation of 3-aminophenol is shown to be 4-aminosalicylic acid. A synthesis of the isomeric 4-hydroxyanthranilic acid is descr: A no. of derivatives of 4-aminosalicylic acid were prepd for test against pathogenic strains...

Roux, J., 1915: Sur les Potamonides qui habitent l'ile de Ceylan. Revue Suisse de Zoologie, 23 361-384

Schroeder, R.; Mundegar, R.R.; Treusch, M.; Schlegel, U.; Bluemcke, I.; Owaribe, K.; Magin, T.M., 1997: Altered distribution of plectin/HD1 in dystrophinopathies. Plectin/HD1 is a high molecular weight protein (approximately 500 kDa) that has been proposed to act as an important and versatile cytoskeletal cross-linker molecule. Mutations of the human plectin gene have recently been associated with the autos...

Gerschitz E.M.; Glick J.A., 1981: The consequences of nominal realism for symbolic mediation. Kindergarten, 5th- and 6th-grade children (total 39 boys and girls) were screened for color blindness and administered a nominal realism questionnaire, which taps the child's understanding of the relationships between words and their referent...

Berg, K., 1932: Ist das Alter der Latenzeier der Daphnien ein geschlechts-bestimmendei; Factor ?. Archiv fuer Hydrobiologie Stuttgart, 24: 497-508

Yoneda, K., 2005: Phosphoglyceric acid mutase, bisphosphoglyceric acid mutase. Nihon Rinsho. Japanese Journal of Clinical Medicine 62 Suppl 11: 463-465

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